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Modulating Myoblast Differentiation with RNA Controllers.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	Modulating Myoblast Differentiation with RNA Controllers./
作者:	Dykstra, Peter Bruce.
面頁冊數:	1 online resource (129 pages)
附註:	Source: Dissertations Abstracts International, Volume: 84-11, Section: B.
Contained By:	Dissertations Abstracts International84-11B.
標題:	Yeast. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30411570click for full text (PQDT)
ISBN:	9798379481674

Modulating Myoblast Differentiation with RNA Controllers.
Dykstra, Peter Bruce.

Modulating Myoblast Differentiation with RNA Controllers. - 1 online resource (129 pages)

Source: Dissertations Abstracts International, Volume: 84-11, Section: B.

Thesis (Ph.D.)--Stanford University, 2022.

Includes bibliographical references

RNA synthetic biology utilizes the key roles of RNA in cellular information transmission and regulation to design and build new biological systems. Engineered RNA devices have been applied to many spheres of biotechnology and human health. One area of human health particularly suited to RNA device applications is regenerative medicine and tissue engineering. Skeletal muscle offers a convenient model system to test RNA device activity through the regenerative capacity of muscle mediated by muscle stem cells and their myoblast progeny. Here, I present my work to design, build, and test RNA devices for the modulation of myoblast differentiation in skeletal muscle. I first discuss the history and current state of synthetic biology, the set of possibilities regenerative medicine offers as an application space, and the latest applications of synthetic biology to mammalian cell fate decisions. Next, I review the field of engineered RNA devices and highlight the ongoing application of RNA devices as well as advances in the development of new RNA aptamers and new mechanisms of cell control using RNA. I then introduce my thesis project where I applied ribozyme-based RNA devices to myoblast differentiation and demonstrated ligand-responsive inhibition of differentiation marker transcript levels as well as inhibition of the downstream fusion of myoblasts into differentiated myotubes. Finally, I offer reflections on my project and suggestions for the design of future RNA-based regulatory systems.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2023

Mode of access: World Wide Web

ISBN: 9798379481674Subjects--Topical Terms:

621059
Yeast.
Index Terms--Genre/Form:

542853
Electronic books.

Modulating Myoblast Differentiation with RNA Controllers.
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