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Optimizing Cyanobacterial Cultivation for Wastewater Nutrient Conversion.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Optimizing Cyanobacterial Cultivation for Wastewater Nutrient Conversion./
Author:	Hasan, Rifat.
Description:	1 online resource (223 pages)
Notes:	Source: Dissertations Abstracts International, Volume: 85-01, Section: B.
Contained By:	Dissertations Abstracts International85-01B.
Subject:	Aquaculture. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30516479click for full text (PQDT)
ISBN:	9798379871963

Optimizing Cyanobacterial Cultivation for Wastewater Nutrient Conversion.
Hasan, Rifat.

Optimizing Cyanobacterial Cultivation for Wastewater Nutrient Conversion. - 1 online resource (223 pages)

Source: Dissertations Abstracts International, Volume: 85-01, Section: B.

Thesis (Ph.D.)--North Carolina State University, 2023.

Includes bibliographical references

Sustainability in livestock and aquaculture wastewater treatment is essential as these sectors continue to grow and the need for alternative treatments is a pressing concern. This research investigates the potential of the cyanobacterial species S. elongatus UTEX 2973 as an alternative sustainable wastewater treatment to 1) remove inorganic nutrients such as ammonia, nitrate, and phosphate from a combination of swine and fish wastewater while simultaneously accumulating carbohydrate during bioremediation, 2) investigate the phosphate and metal removal by abiotic precipitation and S. elongatus assimilation, and 3) predict growth and nutrient removal using a genome-scale model of S. elongatusUTEX 2973 and compare with experimental findings.The first objective was accomplished by selecting a wastewater mixture from three different mixing ratios (25:75, 50:50, and 75:25) of sturgeon and swine wastewater that supports optimal growth and carbohydrate accumulation in S. elongatus biomass. The combination of 50%-50% sturgeon-swine wastewater was selected for further comparison with BG11 synthetic growth media. A higher growth rate was achieved in synthetic growth media, whereas higher carbohydrate accumulation was observed in mixed wastewater. The nutrient removal efficiencies of S. elongatusin the mixed wastewater were 97.98% for ammonia, 93.39% for nitrate-nitrite, and 67.15% for orthophosphate.Significant phosphate removal by abiotic precipitation was observed due to the high pH (~ 9) of wastewater, which led to the second objective, in which both cyanobacterial and abiotic phosphate removal were studied. It was observed that a total of 96.81% phosphate was removed, of which 80.12% occurred by precipitation and 16.69% by S. elongatusassimilation. Within the first week of the two-week experiment ~ 100% precipitation took place.The final objective was accomplished by performing flux balance analysis (FBA) of a genome-scale model of S. elongatus UTEX 2973 with varying environmental parameters such as carbon dioxide (CO2), ammonia, nitrate, and phosphate to optimize either biomass production or glycogen accumulation (major source of carbohydrates in S. elongatus. No accumulation of glycogen was predicted below CO2 thresholds when examined as a function of inorganic nutrients (ammonia, nitrate, and phosphate), below which no accumulation was predicted. Dynamic flux balance analysis (DFBA) was performed using kinetic parameters for nitrate uptake from literature for another cyanobacterial species, Synechocystis sp. PCC6803. The nutrient uptake rates found experimentally did not fit Michaelis-Menten kinetics due to the complexity of wastewater components. Hence, a regression analysis was performed, and a complete second-order polynomial exhibited a good fit (R2=0.99) with experimentally observed nutrient uptake.The findings of this research showed that S. elongatus could bioremediate mixed wastewater by successfully removing inorganic nutrients and accumulating a significant amount of carbohydrates (70-75% of dry cell mass) during the bioremediation process. This particular species has been minimally investigated before for its ability to transform wastewater nutrients into high-value carbohydrate-enriched biomass. The outcomes of this study can support the development of an S. elongatusbiorefinery approach for bioremediation and manufacturing value-added products.

Electronic reproduction.
Ann Arbor, Mich. :
ProQuest,
2023

Mode of access: World Wide Web

ISBN: 9798379871963Subjects--Topical Terms:

545878
Aquaculture.
Index Terms--Genre/Form:

542853
Electronic books.

Optimizing Cyanobacterial Cultivation for Wastewater Nutrient Conversion.
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245 1 0 $a Optimizing Cyanobacterial Cultivation for Wastewater Nutrient Conversion.
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500 $a Source: Dissertations Abstracts International, Volume: 85-01, Section: B.
500 $a Advisor: Beck, Ashley; Hall, Steven.
502 $a Thesis (Ph.D.)--North Carolina State University, 2023.
504 $a Includes bibliographical references
520 $a Sustainability in livestock and aquaculture wastewater treatment is essential as these sectors continue to grow and the need for alternative treatments is a pressing concern. This research investigates the potential of the cyanobacterial species S. elongatus UTEX 2973 as an alternative sustainable wastewater treatment to 1) remove inorganic nutrients such as ammonia, nitrate, and phosphate from a combination of swine and fish wastewater while simultaneously accumulating carbohydrate during bioremediation, 2) investigate the phosphate and metal removal by abiotic precipitation and S. elongatus assimilation, and 3) predict growth and nutrient removal using a genome-scale model of S. elongatusUTEX 2973 and compare with experimental findings.The first objective was accomplished by selecting a wastewater mixture from three different mixing ratios (25:75, 50:50, and 75:25) of sturgeon and swine wastewater that supports optimal growth and carbohydrate accumulation in S. elongatus biomass. The combination of 50%-50% sturgeon-swine wastewater was selected for further comparison with BG11 synthetic growth media. A higher growth rate was achieved in synthetic growth media, whereas higher carbohydrate accumulation was observed in mixed wastewater. The nutrient removal efficiencies of S. elongatusin the mixed wastewater were 97.98% for ammonia, 93.39% for nitrate-nitrite, and 67.15% for orthophosphate.Significant phosphate removal by abiotic precipitation was observed due to the high pH (~ 9) of wastewater, which led to the second objective, in which both cyanobacterial and abiotic phosphate removal were studied. It was observed that a total of 96.81% phosphate was removed, of which 80.12% occurred by precipitation and 16.69% by S. elongatusassimilation. Within the first week of the two-week experiment ~ 100% precipitation took place.The final objective was accomplished by performing flux balance analysis (FBA) of a genome-scale model of S. elongatus UTEX 2973 with varying environmental parameters such as carbon dioxide (CO2), ammonia, nitrate, and phosphate to optimize either biomass production or glycogen accumulation (major source of carbohydrates in S. elongatus. No accumulation of glycogen was predicted below CO2 thresholds when examined as a function of inorganic nutrients (ammonia, nitrate, and phosphate), below which no accumulation was predicted. Dynamic flux balance analysis (DFBA) was performed using kinetic parameters for nitrate uptake from literature for another cyanobacterial species, Synechocystis sp. PCC6803. The nutrient uptake rates found experimentally did not fit Michaelis-Menten kinetics due to the complexity of wastewater components. Hence, a regression analysis was performed, and a complete second-order polynomial exhibited a good fit (R2=0.99) with experimentally observed nutrient uptake.The findings of this research showed that S. elongatus could bioremediate mixed wastewater by successfully removing inorganic nutrients and accumulating a significant amount of carbohydrates (70-75% of dry cell mass) during the bioremediation process. This particular species has been minimally investigated before for its ability to transform wastewater nutrients into high-value carbohydrate-enriched biomass. The outcomes of this study can support the development of an S. elongatusbiorefinery approach for bioremediation and manufacturing value-added products.
533 $a Electronic reproduction. $b Ann Arbor, Mich. : $c ProQuest, $d 2023
538 $a Mode of access: World Wide Web
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650 4 $a Agriculture. $3 518588
650 4 $a Nitrates. $3 914879
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650 4 $a Aquatic sciences. $3 3174300
650 4 $a Biological oceanography. $3 2122748
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710 2 $a North Carolina State University. $3 1018772
773 0 $t Dissertations Abstracts International $g 85-01B.
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=30516479 $z click for full text (PQDT)