東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

A comparative study: Dietary grape ...

Dowdy, Deanna Lynne.

FindBook

Google Book

Amazon

博客來

A comparative study: Dietary grape extracts, derived dietary supplements and the effect of tannins on antioxidant, anticancer, and anti-inflammatory activity.

紀錄類型:	書目-電子資源 : Monograph/item
正題名/作者:	A comparative study: Dietary grape extracts, derived dietary supplements and the effect of tannins on antioxidant, anticancer, and anti-inflammatory activity./
作者:	Dowdy, Deanna Lynne.
面頁冊數:	150 p.
附註:	Source: Dissertation Abstracts International, Volume: 64-07, Section: B, page: 3239.
Contained By:	Dissertation Abstracts International64-07B.
標題:	Chemistry, Agricultural. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3097957
ISBN:	049645570X

A comparative study: Dietary grape extracts, derived dietary supplements and the effect of tannins on antioxidant, anticancer, and anti-inflammatory activity.
Dowdy, Deanna Lynne.

A comparative study: Dietary grape extracts, derived dietary supplements and the effect of tannins on antioxidant, anticancer, and anti-inflammatory activity. - 150 p.

Source: Dissertation Abstracts International, Volume: 64-07, Section: B, page: 3239.

Thesis (Ph.D.)--University of California, Davis, 2003.

The antioxidant, anti-cancer, and anti-inflammatory activities of extracts of red wine, grape juice, whole grapes, grape seeds, grape skins, and GSKE extracts were compared. A portion of each extract was detannified, and both unprocessed and detannified extracts were separated into ten fractions by flash chromatography. Antioxidant activity was assessed spectrophotometrically by measuring the reduction of the 2,2-diphenyl-1-picrylhydrazyl radical. Fractions with antioxidant activity were re-assayed over a range of concentrations to obtain effective concentration (EC50) values for scavenging the DPPH radical, and by thin-layer chromatograpy. Strong antioxidant activity was associated with all the extracts. For red wine and grapes, the presence of tannins was strongly linked to the antioxidant activity. The grape extracts were examined for anti-cancer activity using alamarBlue as an indicator of cell viability. The extracts were not cytotoxic to Jurkat and L929 cancer cells and did not protect Jurkat cells from killing by tumor necrosis factor (TNF). However, several pure compounds---resveratrol, ellagic acid and gallic acid known to be present in red wine---did significantly protect these cells against TNF killing. Although these compounds protected Jurkat cells against TNF, they were not present in sufficient quantities in the extracts to protect the cells. Extracts were screened for anti-inflammatory activity using luciferase reporter gene-transfected JB-6 cells that were either unstimulated or stimulated with trypsin. Unprocessed red wine contained inhibitors of NF-kappa-B transcription. Transcription was inhibited by grape constituents in unstimulated cells, but was stimulated in cells treated with trypsin. Although both inhibitory and stimulatory fractions were present in grape seeds and grape skin, the crude extract of grape seeds did not affect NF-kappa-B transcription. Transcription in the cells was stimulated by several GSKE fractions but not by the crude extract. Further research may confirm that grape extracts are capable of significant biological health effects.

ISBN: 049645570XSubjects--Topical Terms:

1021829
Chemistry, Agricultural.

A comparative study: Dietary grape extracts, derived dietary supplements and the effect of tannins on antioxidant, anticancer, and anti-inflammatory activity.
LDR:03110nmm 2200289 4500 001 1837499
005 20050506072707.5
008 130614s2003 eng d
020 $a 049645570X
035 $a (UnM)AAI3097957
035 $a AAI3097957
040 $a UnM $c UnM
100 1 $a Dowdy, Deanna Lynne. $3 1925946
245 1 2 $a A comparative study: Dietary grape extracts, derived dietary supplements and the effect of tannins on antioxidant, anticancer, and anti-inflammatory activity.
300 $a 150 p.
500 $a Source: Dissertation Abstracts International, Volume: 64-07, Section: B, page: 3239.
500 $a Adviser: Annie Joyce King.
502 $a Thesis (Ph.D.)--University of California, Davis, 2003.
520 $a The antioxidant, anti-cancer, and anti-inflammatory activities of extracts of red wine, grape juice, whole grapes, grape seeds, grape skins, and GSKE extracts were compared. A portion of each extract was detannified, and both unprocessed and detannified extracts were separated into ten fractions by flash chromatography. Antioxidant activity was assessed spectrophotometrically by measuring the reduction of the 2,2-diphenyl-1-picrylhydrazyl radical. Fractions with antioxidant activity were re-assayed over a range of concentrations to obtain effective concentration (EC50) values for scavenging the DPPH radical, and by thin-layer chromatograpy. Strong antioxidant activity was associated with all the extracts. For red wine and grapes, the presence of tannins was strongly linked to the antioxidant activity. The grape extracts were examined for anti-cancer activity using alamarBlue as an indicator of cell viability. The extracts were not cytotoxic to Jurkat and L929 cancer cells and did not protect Jurkat cells from killing by tumor necrosis factor (TNF). However, several pure compounds---resveratrol, ellagic acid and gallic acid known to be present in red wine---did significantly protect these cells against TNF killing. Although these compounds protected Jurkat cells against TNF, they were not present in sufficient quantities in the extracts to protect the cells. Extracts were screened for anti-inflammatory activity using luciferase reporter gene-transfected JB-6 cells that were either unstimulated or stimulated with trypsin. Unprocessed red wine contained inhibitors of NF-kappa-B transcription. Transcription was inhibited by grape constituents in unstimulated cells, but was stimulated in cells treated with trypsin. Although both inhibitory and stimulatory fractions were present in grape seeds and grape skin, the crude extract of grape seeds did not affect NF-kappa-B transcription. Transcription in the cells was stimulated by several GSKE fractions but not by the crude extract. Further research may confirm that grape extracts are capable of significant biological health effects.
590 $a School code: 0029.
650 4 $a Chemistry, Agricultural. $3 1021829
650 4 $a Health Sciences, Toxicology. $3 1017752
650 4 $a Health Sciences, Nutrition. $3 1017801
690 $a 0749
690 $a 0383
690 $a 0570
710 2 0 $a University of California, Davis. $3 1018682
773 0 $t Dissertation Abstracts International $g 64-07B.
790 1 0 $a King, Annie Joyce, $e advisor
790 $a 0029
791 $a Ph.D.
792 $a 2003
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3097957