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DNA reassociation kinetics and devel...

Wyss, Stefanie.

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DNA reassociation kinetics and development of microsatellite libraries in black spruce (Picea mariana) and red spruce (Picea rubens).

Record Type:	Language materials, printed : Monograph/item
Title/Author:	DNA reassociation kinetics and development of microsatellite libraries in black spruce (Picea mariana) and red spruce (Picea rubens)./
Author:	Wyss, Stefanie.
Description:	148 p.
Notes:	Source: Masters Abstracts International, Volume: 45-02, page: 0707.
Contained By:	Masters Abstracts International45-02.
Subject:	Biology, Bioinformatics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=MR20193
ISBN:	9780494201930

DNA reassociation kinetics and development of microsatellite libraries in black spruce (Picea mariana) and red spruce (Picea rubens).
Wyss, Stefanie.

DNA reassociation kinetics and development of microsatellite libraries in black spruce (Picea mariana) and red spruce (Picea rubens). - 148 p.

Source: Masters Abstracts International, Volume: 45-02, page: 0707.

Thesis (M.Sc.)--Laurentian University (Canada), 2006.

Although the genus Picea contains economically important species in Canada that contribute to about 30% of the total Canadian Forest production, genetic research in this genus has lagged behind that of other important plant species. Conifer species such as spruces are characterized by their large genome size whose organization is still largely unknown. The cot analysis or DNA reassociation kinetics can shed information on the genome size and the percentage of low, intermediate and high copy DNA. The information from cot analysis would be useful in the development of low copy microsatellite libraries. Attempts were made to generate a DNA reassociation curve for black spruce (Picea mariana) and red spruce (Picea rubens ) using cot analysis and hydroxyapatite chromatography. The GC content for red spruce was estimated at approximately 45% and at 50% for black spruce. The DNA from red spruce reached 75% reassociation whereas for black spruce, the percentage of reassociation was not conclusive. The percentage of high copy DNA was determined between 23 and 27% for Picea rubens. Attempts to develop microsatellite enriched DNA libraries prior to developing low copy microsatellite were more successful with black spruce DNA than with red spruce DNA. Several hundreds of positive clones were identified in the black spruce libraries and seven of them were characterized. Two microsatellites were perfect, two imperfect and three were compound microsatellites. Some primer pairs designed from these microsatellites generated polymorphic bands when used to amplify DNA from individual black spruce genotypes.

ISBN: 9780494201930Subjects--Topical Terms:

1018415
Biology, Bioinformatics.

DNA reassociation kinetics and development of microsatellite libraries in black spruce (Picea mariana) and red spruce (Picea rubens).
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100 1 $a Wyss, Stefanie. $3 1296394
245 1 0 $a DNA reassociation kinetics and development of microsatellite libraries in black spruce (Picea mariana) and red spruce (Picea rubens).
300 $a 148 p.
500 $a Source: Masters Abstracts International, Volume: 45-02, page: 0707.
502 $a Thesis (M.Sc.)--Laurentian University (Canada), 2006.
520 $a Although the genus Picea contains economically important species in Canada that contribute to about 30% of the total Canadian Forest production, genetic research in this genus has lagged behind that of other important plant species. Conifer species such as spruces are characterized by their large genome size whose organization is still largely unknown. The cot analysis or DNA reassociation kinetics can shed information on the genome size and the percentage of low, intermediate and high copy DNA. The information from cot analysis would be useful in the development of low copy microsatellite libraries. Attempts were made to generate a DNA reassociation curve for black spruce (Picea mariana) and red spruce (Picea rubens ) using cot analysis and hydroxyapatite chromatography. The GC content for red spruce was estimated at approximately 45% and at 50% for black spruce. The DNA from red spruce reached 75% reassociation whereas for black spruce, the percentage of reassociation was not conclusive. The percentage of high copy DNA was determined between 23 and 27% for Picea rubens. Attempts to develop microsatellite enriched DNA libraries prior to developing low copy microsatellite were more successful with black spruce DNA than with red spruce DNA. Several hundreds of positive clones were identified in the black spruce libraries and seven of them were characterized. Two microsatellites were perfect, two imperfect and three were compound microsatellites. Some primer pairs designed from these microsatellites generated polymorphic bands when used to amplify DNA from individual black spruce genotypes.
590 $a School code: 1100.
650 4 $a Biology, Bioinformatics. $3 1018415
690 $a 0715
710 2 0 $a Laurentian University (Canada). $3 1019463
773 0 $t Masters Abstracts International $g 45-02.
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