東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

Physicochemical characterization of ...

Selvi, Bilge.

FindBook

Google Book

Amazon

博客來

Physicochemical characterization of camptothecin membrane binding properties and polymeric microsphere formulations.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Physicochemical characterization of camptothecin membrane binding properties and polymeric microsphere formulations./
作者:	Selvi, Bilge.
面頁冊數:	127 p.
附註:	Adviser: Michalakis Savva.
Contained By:	Dissertation Abstracts International68-12B.
標題:	Chemistry, Pharmaceutical. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3293118
ISBN:	9780549368649

Physicochemical characterization of camptothecin membrane binding properties and polymeric microsphere formulations.
Selvi, Bilge.

Physicochemical characterization of camptothecin membrane binding properties and polymeric microsphere formulations. - 127 p.

Adviser: Michalakis Savva.

Thesis (Ph.D.)--Long Island University, The Brooklyn Center, 2008.

The results indicate that the stabilization and sustained release of CPT from H-PLGA microspheres might reduce local toxicity while simultaneously prolonging efficiency, suggesting new perspectives in CPT chemotherapy.

ISBN: 9780549368649Subjects--Topical Terms:

550957
Chemistry, Pharmaceutical.

Physicochemical characterization of camptothecin membrane binding properties and polymeric microsphere formulations.
LDR:04379nam 2200349 a 45 001 962478
005 20110830
008 110831s2008 ||||||||||||||||| ||eng d
020 $a 9780549368649
035 $a (UMI)AAI3293118
035 $a AAI3293118
040 $a UMI $c UMI
100 1 $a Selvi, Bilge. $3 1285534
245 1 0 $a Physicochemical characterization of camptothecin membrane binding properties and polymeric microsphere formulations.
300 $a 127 p.
500 $a Adviser: Michalakis Savva.
500 $a Source: Dissertation Abstracts International, Volume: 68-12, Section: B, page: 8043.
502 $a Thesis (Ph.D.)--Long Island University, The Brooklyn Center, 2008.
520 $a The results indicate that the stabilization and sustained release of CPT from H-PLGA microspheres might reduce local toxicity while simultaneously prolonging efficiency, suggesting new perspectives in CPT chemotherapy.
520 $a In an effort to design novel formulation strategies to optimize the antitumor activity of camptothecin (CPT), the physicochemical and membrane binding properties of the drug, were investigated by various techniques in acidic and physiological pH. The intrinsic solubility of the CPT-lactone free base was determined to be 3.44 muM and 5.11 muM at 22°C and 37°C, respectively. The equilibrium solubility of the drug was found to increase with increasing temperature and decreasing pH. The enhanced solubility of the drug at very low pH is attributed to the protonation of the nitrogen atom in the ring B and the increased solvency of the highly acidic media. The logarithmic value of the intrinsic partition coefficient P of the free base CPT-lactone form was estimated to be 1.65, characteristic of a molecule suitable for oral absorption.
520 $a The association constants Kf of the drug for bilayers composed of the zwitterionic 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and the negatively-charged 1,2-dioleoyl-sn-glycero-3-phospho- rac-(1-glycerol) (DOPG) were studied at acidic pH by fluorescence anisotropy and determined to be 35.4 +/- 4.5 M-1 and 93.1 +/- 11.0 M-1 for DOPC and DOPG, respectively, indicating a tendency of CPT to preferentially bind to negatively charged membranes.
520 $a The energy of activation for the hydrolysis of CPT at physiological pH was found to be 114.3 +/- 33.4 kj/mole. The calculated t½ of the reaction at pH 7.2 at temperatures 25°C and 10°C was found to be 0.07 days and 5.12 days, respectively, whereas the time required for 1% of CPT-lactone to hydrolyze to CPT-carboxylate (t99%) was determined to be 1.8 hours, thus offering enough time to safely handle CPT-lactone at low temperatures.
520 $a The preformulation results indicated that at highly acidic media CPT is positively charged and exists at its stable lactone form of increased solubility and has a capacity to bind to negatively charged membranes. Taking advantage of the increased stability of CPT in acidic media CPT-loaded microspheres were prepared in a 10 N HCl-methylene chloride mixture using the H-series of poly(D,L-lactide-co-glycolide) (H-PLGA). The system was then compared with a standard microsphere formation method and the results were evaluated with respect to particle morphology and drug release profile.
520 $a Rough surface of the particles were obtained from the preparation method where a 10 N HCl solution was used. The release pattern of CPT was biphasic comprising a first burst effect followed by zero order release for all the formulations. However, the release of the drug was slightly faster from the microspheres formed with the modified method compared to the standard.
520 $a Until now clinical application of CPT has been highly restricted by the insolubility and instability of the drug in its active lactone form, resulting in less antitumor potency and poor bioavailability. The pH-dependent release of the CPT-loaded microspheres was also compared and faster initial release (burst phase) was found at neutral pH, whereas at low pH the release was zero order for all the formulations.
590 $a School code: 0198.
650 4 $a Chemistry, Pharmaceutical. $3 550957
650 4 $a Physics, Molecular. $3 1018648
690 $a 0491
690 $a 0609
710 2 $a Long Island University, The Brooklyn Center. $3 1017735
773 0 $t Dissertation Abstracts International $g 68-12B.
790 $a 0198
790 1 0 $a Savva, Michalakis, $e advisor
791 $a Ph.D.
792 $a 2008
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3293118