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From crabs to hamsters: Bioanalytica...

The University of Wisconsin - Madison.

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From crabs to hamsters: Bioanalytical mass spectrometry for peptidomic analysis and biomarker discovery.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	From crabs to hamsters: Bioanalytical mass spectrometry for peptidomic analysis and biomarker discovery./
作者:	Schmidt, Joshua John.
面頁冊數:	302 p.
附註:	Adviser: Lingjun Li.
Contained By:	Dissertation Abstracts International68-08B.
標題:	Biology, Bioinformatics. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3279009
ISBN:	9780549190899

From crabs to hamsters: Bioanalytical mass spectrometry for peptidomic analysis and biomarker discovery.
Schmidt, Joshua John.

From crabs to hamsters: Bioanalytical mass spectrometry for peptidomic analysis and biomarker discovery. - 302 p.

Adviser: Lingjun Li.

Thesis (Ph.D.)--The University of Wisconsin - Madison, 2007.

While sometimes overlooked, peptidomics and peptide diversity is one of the most important areas of research today. This is because of the vital role that naturally created peptides play in cellular and systemic communication. Peptides have been shown to control and regulate many physiological processes, including feeding, sleep, development, and even cell death. Currently there is a lack of knowledge as to the peptide content of different but related species, as well as peptide distribution throughout nervous system within the same species. Additionally, due to integrative activity of bioactive peptides in physiological processes, there is also great potential for their use as biomarkers to indicate disease infection or progression. To begin filling in these gaps in knowledge, mass spectrometric (MS) methods have been developed for both peptidomic analysis and biomarker identification. Specifically, matrix-assisted laser desorption/ionization (MALDI) Fourier transform mass spectrometry has been used for a majority of this work due to its sensitivity, high mass measurement accuracy, and high resolution. A MALDI time-of-flight/time-of-flight instrument was also used for tissue-specific peptidomic profiling due to its sensitivity and high throughput nature. Furthermore, as the application of these methods produced a large set of data, bioinformatics methods were developed and used to process and analyze the results of MS analysis. The combination of mass spectrometry with bioinformatics has enabled rapid comparison of peptidomes of five brachyuran crabs and the determination of peptide distribution among the model nervous system and neurohemal organs of the crustacean C. borealis. Furthermore, this approach also allowed the discovery of a panel of classifying features (biomarkers) from prion infected hamsters useful in identifying infected animals. Overall, this research has developed improved methodologies and tools for peptidomic analysis and demonstrated their utility in neurochemical signaling and biomarker discovery in biological fluids.

ISBN: 9780549190899Subjects--Topical Terms:

1018415
Biology, Bioinformatics.

From crabs to hamsters: Bioanalytical mass spectrometry for peptidomic analysis and biomarker discovery.
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