東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Bioinformatics tool development and ...

Staton, Margaret E.

Linked to FindBook

Google Book

Amazon

博客來

Bioinformatics tool development and sequence analysis of Rosaceae family expressed sequence tags.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Bioinformatics tool development and sequence analysis of Rosaceae family expressed sequence tags./
Author:	Staton, Margaret E.
Description:	191 p.
Notes:	Adviser: Doreen Main.
Contained By:	Dissertation Abstracts International68-07B.
Subject:	Biology, Bioinformatics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3274290
ISBN:	9780549149620

Bioinformatics tool development and sequence analysis of Rosaceae family expressed sequence tags.
Staton, Margaret E.

Bioinformatics tool development and sequence analysis of Rosaceae family expressed sequence tags. - 191 p.

Adviser: Doreen Main.

Thesis (Ph.D.)--Clemson University, 2007.

Background. An international community of researchers has generated a significant number of Expressed Sequence Tags (ESTs) for the Rosaceae, an economically important plant family that includes most temperate fruits such as apple, cherry, peach, and strawberry as well as other commercially valuable members. ESTs are fragments of expressed genes that can be used for gene discovery, developing markers for mapping and cultivar improvement via marker assisted selection.

ISBN: 9780549149620Subjects--Topical Terms:

1018415
Biology, Bioinformatics.

Bioinformatics tool development and sequence analysis of Rosaceae family expressed sequence tags.
LDR:02974nam 2200289 a 45 001 947660
005 20110524
008 110524s2007 ||||||||||||||||| ||eng d
020 $a 9780549149620
035 $a (UMI)AAI3274290
035 $a AAI3274290
040 $a UMI $c UMI
100 1 $a Staton, Margaret E. $3 1271131
245 1 0 $a Bioinformatics tool development and sequence analysis of Rosaceae family expressed sequence tags.
300 $a 191 p.
500 $a Adviser: Doreen Main.
500 $a Source: Dissertation Abstracts International, Volume: 68-07, Section: B, page: 4209.
502 $a Thesis (Ph.D.)--Clemson University, 2007.
520 $a Background. An international community of researchers has generated a significant number of Expressed Sequence Tags (ESTs) for the Rosaceae, an economically important plant family that includes most temperate fruits such as apple, cherry, peach, and strawberry as well as other commercially valuable members. ESTs are fragments of expressed genes that can be used for gene discovery, developing markers for mapping and cultivar improvement via marker assisted selection.
520 $a Description. The Genome Database for Rosaceae (GDR) was initiated to provide a curated and integrated web-based relational database for this family. I developed a key component of GDR to assemble and annotate the publicly available ESTs from the four main genera of the family ( Prunus, Malus, Fragaria, Rosa). I created both genera and family level unigenes using the software CAP3 after extensive filtering, trimming and assembly. Further analysis includes marker mining for single nucleotide polymorphisms (SNPs) and simple sequence repeats (SSRs) with putative primer identification, and oligo identification for potential microarray development. Functional genomics efforts are supported with sequence similarity searching against major protein and nucleotide databases, gene product ontology assignment, and protein motif identification. I deployed the entire project on the GDR with all data available for browsing, searching, and downloading.
520 $a Conclusions. The GDR and its associated EST unigene project are meeting a major need for timely annotation and curation of sequence data for the Rosaceae community. The results of my analysis highlight major genes and pathways of interest including ripening, disease resistance, and transcription factors. The easily accessible pool of annotated coding sequences should further both functional and structural genomics characterization in Rosaceae. The unigene elucidates the levels of sequence similarity shared across different plant species and the implications for resource sharing across the family. GDR can be accessed at http://www.rosaceae.org/.
590 $a School code: 0050.
650 4 $a Biology, Bioinformatics. $3 1018415
690 $a 0715
710 2 $a Clemson University. $b Environment. $3 1271132
773 0 $t Dissertation Abstracts International $g 68-07B.
790 $a 0050
790 1 0 $a Main, Doreen, $e advisor
791 $a Ph.D.
792 $a 2007
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3274290