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Bone protective effects of dried plu...

Bu, So Young.

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Bone protective effects of dried plum and its polyphenols under inflammatory and oxidative stress conditions.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Bone protective effects of dried plum and its polyphenols under inflammatory and oxidative stress conditions./
Author:	Bu, So Young.
Description:	216 p.
Notes:	Adviser: Brenda J. Smith.
Contained By:	Dissertation Abstracts International68-04B.
Subject:	Health Sciences, Nutrition. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3259592

Bone protective effects of dried plum and its polyphenols under inflammatory and oxidative stress conditions.
Bu, So Young.

Bone protective effects of dried plum and its polyphenols under inflammatory and oxidative stress conditions. - 216 p.

Adviser: Brenda J. Smith.

Thesis (Ph.D.)--Oklahoma State University, 2007.

Scope and method of study. The purpose of this study was to utilize the combination of in vivo and in vitro studies to investigate the effects of dried plum (DP) on bone and bone cell activity under conditions of elevated pro-inflammatory mediators. In Study 1, 9-month-old male osteopenic orchidectomized rats were randomly assigned to control (AIN-93M) or DP diet (25%, w/w) for 90 days. The positive control group consumed the control diet and received PTH (80mug/kg). In Study 2, macrophages (RAW 264.7) were pretreated with the polyphenols extracted from DP (0, 10, 20, or 30 mug/ml) cultured with or without RANKL and then stimulated with either LPS or hydrogen peroxide (H2O2). TRAP-positive osteoclasts and resorption pit formation were evaluated and inflammatory mediators such as NO and TNF-alpha by macrophages and during their differentiation into osteoclasts were assessed. In Study 3, MC3T3-E1 cells, murine pre-osteoblasts, were utilized to determine how DP polyphenols influence osteoblast activity, function and signaling under normal and inflammatory conditions. Cells were pretreated with polyphenols (0, 2.5, 5, 10 and 20 mug/ml) prior to stimulation with TNF-alpha (0 or 1.0 ng/ml). ALP activity and mineralized nodule formation were measured and alterations in gene expression of RANKL, OPG, Runx2, Osterix and IGF-I were analyzed.Subjects--Topical Terms:

1017801
Health Sciences, Nutrition.

Bone protective effects of dried plum and its polyphenols under inflammatory and oxidative stress conditions.
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005 20110519
008 110519s2007 eng d
035 $a (UMI)AAI3259592
035 $a AAI3259592
040 $a UMI $c UMI
100 1 $a Bu, So Young. $3 1265618
245 1 0 $a Bone protective effects of dried plum and its polyphenols under inflammatory and oxidative stress conditions.
300 $a 216 p.
500 $a Adviser: Brenda J. Smith.
500 $a Source: Dissertation Abstracts International, Volume: 68-04, Section: B, page: 2261.
502 $a Thesis (Ph.D.)--Oklahoma State University, 2007.
520 $a Scope and method of study. The purpose of this study was to utilize the combination of in vivo and in vitro studies to investigate the effects of dried plum (DP) on bone and bone cell activity under conditions of elevated pro-inflammatory mediators. In Study 1, 9-month-old male osteopenic orchidectomized rats were randomly assigned to control (AIN-93M) or DP diet (25%, w/w) for 90 days. The positive control group consumed the control diet and received PTH (80mug/kg). In Study 2, macrophages (RAW 264.7) were pretreated with the polyphenols extracted from DP (0, 10, 20, or 30 mug/ml) cultured with or without RANKL and then stimulated with either LPS or hydrogen peroxide (H2O2). TRAP-positive osteoclasts and resorption pit formation were evaluated and inflammatory mediators such as NO and TNF-alpha by macrophages and during their differentiation into osteoclasts were assessed. In Study 3, MC3T3-E1 cells, murine pre-osteoblasts, were utilized to determine how DP polyphenols influence osteoblast activity, function and signaling under normal and inflammatory conditions. Cells were pretreated with polyphenols (0, 2.5, 5, 10 and 20 mug/ml) prior to stimulation with TNF-alpha (0 or 1.0 ng/ml). ALP activity and mineralized nodule formation were measured and alterations in gene expression of RANKL, OPG, Runx2, Osterix and IGF-I were analyzed.
520 $a Findings and conclusions. Study 1 showed that dietary supplementation with DP restored the ORX-induced loss of bone mass and deterioration of bone structure. The restoration of bone microarchitecture by dried plum was comparable to PTH. Study 2 revealed that DP polyphenols inhibit osteoclastogenesis and osteoclast activity which may be mediated by the down-regulation of cytokines such as TNF-alpha and NO. Study 3 showed that DP polyphenols enhance osteoblast activity and mineralized nodule formation under normal and inflammatory conditions. Results in vitro studies suggest that dried plum which has ability to down-regulate inflammatory mediators, has potent effects on osteoclast and osteoblast activity and provide possible mechanisms by which dried plum preserves bone mass and microarchitecture as reported in current and previous in vivo studies.
590 $a School code: 0664.
650 4 $a Health Sciences, Nutrition. $3 1017801
690 $a 0570
710 2 0 $a Oklahoma State University. $3 626630
773 0 $t Dissertation Abstracts International $g 68-04B.
790 $a 0664
790 1 0 $a Smith, Brenda J., $e advisor
791 $a Ph.D.
792 $a 2007
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3259592