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Statistical methods in experimental ...

Temple University.

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Statistical methods in experimental design and analysis of microarray data.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Statistical methods in experimental design and analysis of microarray data./
Author:	Ding, Yu.
Description:	97 p.
Notes:	Adviser: Damaraju Raghavarao.
Contained By:	Dissertation Abstracts International69-01B.
Subject:	Biology, Bioinformatics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3300345
ISBN:	9780549445647

Statistical methods in experimental design and analysis of microarray data.
Ding, Yu.

Statistical methods in experimental design and analysis of microarray data. - 97 p.

Adviser: Damaraju Raghavarao.

Thesis (Ph.D.)--Temple University, 2008.

In this thesis, we concentrate on statistical methods in identifying differentially expressed genes and providing experimental designs for cDNA microarray experiments.

ISBN: 9780549445647Subjects--Topical Terms:

1018415
Biology, Bioinformatics.

Statistical methods in experimental design and analysis of microarray data.
LDR:03217nmm 2200301 a 45 001 867065
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008 100802s2008 ||||||||||||||||| ||eng d
020 $a 9780549445647
035 $a (UMI)AAI3300345
035 $a AAI3300345
040 $a UMI $c UMI
100 1 $a Ding, Yu. $3 1035768
245 1 0 $a Statistical methods in experimental design and analysis of microarray data.
300 $a 97 p.
500 $a Adviser: Damaraju Raghavarao.
500 $a Source: Dissertation Abstracts International, Volume: 69-01, Section: B, page: 0396.
502 $a Thesis (Ph.D.)--Temple University, 2008.
520 $a In this thesis, we concentrate on statistical methods in identifying differentially expressed genes and providing experimental designs for cDNA microarray experiments.
520 $a Identifying differentially expressed genes for different conditions is one of the main objectives in eDNA microarray experiments. Many current statistical models cannot be used for insufficient replicated arrays. In Chapter 3, we propose a, new method to solve this problem, when a single array data are available. If the log-transformed ratios for the expressed genes as well as unexpressed genes have equal variances, we use a Hadamard matrix to construct a t test from a single array data. Basically, we test whether each doubtful gene has significantly differential expression compared to the unexpressed genes. We form some new random variables corresponding to the rows of a Hadamard matrix using the algebraic sum of gene expressions. A one-sample t test is constructed and the p-value is calculated for each doubtful gene based on these random variables. By using any method for multiple testing, adjusted p-values could be obtained from original p-values and significance of doubtful genes can be determined. When the variance of expressed genes differs from the variance of unexpressed genes, we construct a confidence interval for the difference in the log expression levels and using this interval, we determine differentially expressed genes. In Chapter 4, we generalize the results of Chapter 3 by considering fewer replications of arrays. We use data from doubtfully expressed genes and unexpressed genes to construct a t test, using Satterthwaite's degrees of freedom to identify differentially expressed genes for both direct and indirect designs.
520 $a A good statistical design is important to get valid inferences in microarray experiments. Sonic optimal designs have been proposed in the literature, but they are not widely applicable. In Chapter 5, we discuss designing 2 m factorial cDNA mieroarray experiments using Hadamard matrices. We balance the levels of the factors on the disease conditions and construct orthogonal factorial experiments, in general. These designs orthogonally estimate the effects of interest. In Chapter 6, we extend our work to construct new factorial designs with 3 levels for each factor.
590 $a School code: 0225.
650 4 $a Biology, Bioinformatics. $3 1018415
650 4 $a Statistics. $3 517247
690 $a 0463
690 $a 0715
710 2 $a Temple University. $3 959342
773 0 $t Dissertation Abstracts International $g 69-01B.
790 $a 0225
790 1 0 $a Raghavarao, Damaraju, $e advisor
791 $a Ph.D.
792 $a 2008
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3300345