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Bioinformatics-driven protein design.

Rensselaer Polytechnic Institute.

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Bioinformatics-driven protein design.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Bioinformatics-driven protein design./
Author:	Reeder, Philippa Jane.
Description:	134 p.
Notes:	Advisers: Jonathan S. Dordick; Christopher Bystroff.
Contained By:	Dissertation Abstracts International70-09B.
Subject:	Biology, Bioinformatics. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3371296
ISBN:	9781109371284

Bioinformatics-driven protein design.
Reeder, Philippa Jane.

Bioinformatics-driven protein design. - 134 p.

Advisers: Jonathan S. Dordick; Christopher Bystroff.

Thesis (Ph.D.)--Rensselaer Polytechnic Institute, 2009.

The focus of this thesis is the development of bioinformatics - and rational design-based methods for stabilization and optimization of proteins for use under industrial conditions, as well as in aide of unique design applications. To this end, two approaches are described: the consensus approach, which is used to determine thermostabilizing mutations in the Subtilase superfamily based on protein sequence data mining and rational design, and the re-wire approach, a novel protein redesign methodology under development by our research groups. The primary focus of this thesis, the re-wire method allows direct manipulation of protein structures for optimization of protein folding rates, stability and specificity. In particular, we focus on how changing the topology of a model protein, Green Fluorescent Protein (GFP), without altering core intramolecular contacts effects successful protein folding and stability. In addition, we developed self-reporting polymer-enzyme composite materials for biosensing devices based of the leave-one-out GFP biosensor complementation system.

ISBN: 9781109371284Subjects--Topical Terms:

1018415
Biology, Bioinformatics.

Bioinformatics-driven protein design.
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100 1 $a Reeder, Philippa Jane. $3 1035531
245 1 0 $a Bioinformatics-driven protein design.
300 $a 134 p.
500 $a Advisers: Jonathan S. Dordick; Christopher Bystroff.
500 $a Source: Dissertation Abstracts International, Volume: 70-09, Section: B, page: .
502 $a Thesis (Ph.D.)--Rensselaer Polytechnic Institute, 2009.
520 $a The focus of this thesis is the development of bioinformatics - and rational design-based methods for stabilization and optimization of proteins for use under industrial conditions, as well as in aide of unique design applications. To this end, two approaches are described: the consensus approach, which is used to determine thermostabilizing mutations in the Subtilase superfamily based on protein sequence data mining and rational design, and the re-wire approach, a novel protein redesign methodology under development by our research groups. The primary focus of this thesis, the re-wire method allows direct manipulation of protein structures for optimization of protein folding rates, stability and specificity. In particular, we focus on how changing the topology of a model protein, Green Fluorescent Protein (GFP), without altering core intramolecular contacts effects successful protein folding and stability. In addition, we developed self-reporting polymer-enzyme composite materials for biosensing devices based of the leave-one-out GFP biosensor complementation system.
590 $a School code: 0185.
650 4 $a Biology, Bioinformatics. $3 1018415
650 4 $a Engineering, Chemical. $3 1018531
690 $a 0542
690 $a 0715
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773 0 $t Dissertation Abstracts International $g 70-09B.
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790 1 0 $a Bystroff, Christopher, $e advisor
790 1 0 $a Dordick, Jonathan S., $e advisor
791 $a Ph.D.
792 $a 2009
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3371296