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Role of digestion, iron and protein ...

Joseph, Shama Vasanthi.

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Role of digestion, iron and protein on the antioxidant effect of green and black tea in Caco2 cells.

Record Type:	Electronic resources : Monograph/item
Title/Author:	Role of digestion, iron and protein on the antioxidant effect of green and black tea in Caco2 cells./
Author:	Joseph, Shama Vasanthi.
Description:	67 p.
Notes:	Source: Masters Abstracts International, Volume: 41-04, page: 1061.
Contained By:	Masters Abstracts International41-04.
Subject:	Health Sciences, Nutrition. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1411941
ISBN:	049395340X

Role of digestion, iron and protein on the antioxidant effect of green and black tea in Caco2 cells.
Joseph, Shama Vasanthi.

Role of digestion, iron and protein on the antioxidant effect of green and black tea in Caco2 cells. - 67 p.

Source: Masters Abstracts International, Volume: 41-04, page: 1061.

Thesis (M.S.)--Michigan State University, 2002.

Tea made from the leaves of Camellia sinensis has been vigorously investigated for its antioxidant and potential anti-cancer effects. Most studies have shown beneficial effects of its purified extracts or individual constituents. Evidence on the influence of digestion and other dietary components during digestion is sparse. The aim of this study was to elucidate the effect of simulated digestion on the antioxidant effect of green and black tea and the influence of iron and protein during this process. Caco2 cells were pre-treated for 1 h with either a black or green tea decoction (4 g in 50 ml boiling water for 5 min), digested tea decoction, or tea digested with cereal or with milk and then treated with 500 muM hydrogen peroxide (H2O2). DNA damage was estimated as single strand breaks (Comet assay) and as 8-OHdG formation. Pre-treatment with green tea decoction significantly reduced the percentage of comets from 52 to 36% in the comet assay. However. H2O 2 treatment did not cause significant level of DNA damage in the 8-OHdG assay compared to untreated cells (control) thus making it difficult to make any conclusions related to a protective effect of pre-treatment with tea or tea digest. Neither green nor black tea caused a significant change in 8-OHdG content of cells compared to H2O2 treatment alone. The digestive process had no effect nor did the addition of cereal or milk, except for an apparent pro-oxidant effect with the addition of milk to green tea. Milk and cereal interacted differently with green and black teas as shown by significantly higher concentrations of 8-OHdG when each of these foods was digested with green tea compared to black tea.

ISBN: 049395340XSubjects--Topical Terms:

1017801
Health Sciences, Nutrition.

Role of digestion, iron and protein on the antioxidant effect of green and black tea in Caco2 cells.
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245 1 0 $a Role of digestion, iron and protein on the antioxidant effect of green and black tea in Caco2 cells.
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500 $a Adviser: Mark Ubersax.
502 $a Thesis (M.S.)--Michigan State University, 2002.
520 $a Tea made from the leaves of Camellia sinensis has been vigorously investigated for its antioxidant and potential anti-cancer effects. Most studies have shown beneficial effects of its purified extracts or individual constituents. Evidence on the influence of digestion and other dietary components during digestion is sparse. The aim of this study was to elucidate the effect of simulated digestion on the antioxidant effect of green and black tea and the influence of iron and protein during this process. Caco2 cells were pre-treated for 1 h with either a black or green tea decoction (4 g in 50 ml boiling water for 5 min), digested tea decoction, or tea digested with cereal or with milk and then treated with 500 muM hydrogen peroxide (H2O2). DNA damage was estimated as single strand breaks (Comet assay) and as 8-OHdG formation. Pre-treatment with green tea decoction significantly reduced the percentage of comets from 52 to 36% in the comet assay. However. H2O 2 treatment did not cause significant level of DNA damage in the 8-OHdG assay compared to untreated cells (control) thus making it difficult to make any conclusions related to a protective effect of pre-treatment with tea or tea digest. Neither green nor black tea caused a significant change in 8-OHdG content of cells compared to H2O2 treatment alone. The digestive process had no effect nor did the addition of cereal or milk, except for an apparent pro-oxidant effect with the addition of milk to green tea. Milk and cereal interacted differently with green and black teas as shown by significantly higher concentrations of 8-OHdG when each of these foods was digested with green tea compared to black tea.
590 $a School code: 0128.
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