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Interactions of N-alkyl-2-pyrrolidin...

Kopkalli, Yasemin.

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Interactions of N-alkyl-2-pyrrolidinone surfactants with DMPC bilayers.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Interactions of N-alkyl-2-pyrrolidinone surfactants with DMPC bilayers./
作者:	Kopkalli, Yasemin.
面頁冊數:	211 p.
附註:	Adviser: Lesley Davenport.
Contained By:	Dissertation Abstracts International67-08B.
標題:	Biophysics, General. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3231940
ISBN:	9780542845079

Interactions of N-alkyl-2-pyrrolidinone surfactants with DMPC bilayers.
Kopkalli, Yasemin.

Interactions of N-alkyl-2-pyrrolidinone surfactants with DMPC bilayers. - 211 p.

Adviser: Lesley Davenport.

Thesis (Ph.D.)--City University of New York, 2006.

ITC data reveals an endothermic association for both C8P and C2,6P together with a somewhat higher binding affinity [(6.78+/-0.92)x10 -4M] for C8P compared with C2,6P [(8.46+/-0.83)x10 -4 M], as predicted from fluorescence studies.

ISBN: 9780542845079Subjects--Topical Terms:

1019105
Biophysics, General.

Interactions of N-alkyl-2-pyrrolidinone surfactants with DMPC bilayers.
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100 1 $a Kopkalli, Yasemin. $3 1296257
245 1 0 $a Interactions of N-alkyl-2-pyrrolidinone surfactants with DMPC bilayers.
300 $a 211 p.
500 $a Adviser: Lesley Davenport.
500 $a Source: Dissertation Abstracts International, Volume: 67-08, Section: B, page: 4440.
502 $a Thesis (Ph.D.)--City University of New York, 2006.
520 $a ITC data reveals an endothermic association for both C8P and C2,6P together with a somewhat higher binding affinity [(6.78+/-0.92)x10 -4M] for C8P compared with C2,6P [(8.46+/-0.83)x10 -4 M], as predicted from fluorescence studies.
520 $a There is great interest in identifying and characterizing new surfactants with predictable solubilization and reconstitution characteristics for use in membrane research. Among them, Triton X-100 and octyl glucoside (OG) are the best-studied surfactants. However, no physical data is available characterizing the interactions of two non-ionic pyrrolidinone surfactants, N-(2-ethylhexyl)-2-pyrrolidinone (C2,6P) and N-octyl-2pyrrolidinone (C8P), with lipid bilayers. In the present study, their physical effects on dimyristoylphosphatidylcholine small unilamellar vesicles (DMPC SUVs) above the lipid phase transition temperature are described using surface tension, fluorescence spectroscopy, and isothermal titration calorimetry (ITC) methodologies.
520 $a Surface tension methods show significant deviations from ideal behavior (attractive interactions) for these surfactant/lipid mixtures. In addition, fluorescence intensity studies of DPH and TMA-DPH labeled SUVs suggest that C8P interacts more favorably with both the acyl chain and head group regions of the bilayer over the corresponding branched chain C2,6P analogue. This is further supported by fluoresecence emission anisotropy measurements (EA). Both surfactants affect the membrane lipid order within the acyl chain region, and the corresponding phospholipid 'melt' transition temperature (Tm ) values, which decrease linearly with increasing surfactant concentrations. Using the van't Hoff model, the lipid bilayer-water partition coefficients for these surfactants have been estimated from the depression of the lipid phase transition temperature. Values for the lipid partition coefficient for C8P [(1.22+/-0.08)x104] versus C2,6P [(4.09+/-0.78)x10 3] further supports a more favorable bilayer association for C8P.
520 $a We propose that C8P interacts and affects the lipid packing of bilayer membranes to a greater extent than C2,6P. Interactions of C2,6P appears to be more localized within the head group region of the bilayer. We conclude that C8P has great potential for biological applications, particularly membrane solubilization and membrane formation from micellar mixtures. In contrast, C2,6P may have potential for solubilization of extrinsic surface associated membrane proteins.
590 $a School code: 0046.
650 4 $a Biophysics, General. $3 1019105
650 4 $a Chemistry, Physical. $3 560527
690 $a 0494
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710 2 0 $a City University of New York. $3 1018111
773 0 $t Dissertation Abstracts International $g 67-08B.
790 $a 0046
790 1 0 $a Davenport, Lesley, $e advisor
791 $a Ph.D.
792 $a 2006
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3231940