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Interaction between poloxamers and c...

Wu, Guohui.

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Interaction between poloxamers and cell membranes.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Interaction between poloxamers and cell membranes./
Author:	Wu, Guohui.
Description:	216 p.
Notes:	Adviser: Ka Yee C. Lee.
Contained By:	Dissertation Abstracts International66-03B.
Subject:	Biophysics, General. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3168415
ISBN:	9780542045035

Interaction between poloxamers and cell membranes.
Wu, Guohui.

Interaction between poloxamers and cell membranes. - 216 p.

Adviser: Ka Yee C. Lee.

Thesis (Ph.D.)--The University of Chicago, 2005.

Poloxamers, a family of PEO-PPO-PEO triblock copolymers, are known to help seal electroporated cell membranes. However, the interaction mechanism between the cell membrane and poloxamer is not well understood. Using both phospholipid monolayers and bilayers to mimic cell membranes, we have examined the lipid/poloxamer interaction between poloxamer and cell membranes.

ISBN: 9780542045035Subjects--Topical Terms:

1019105
Biophysics, General.

Interaction between poloxamers and cell membranes.
LDR:02953nam 2200301 a 45 001 972071
005 20110927
008 110927s2005 eng d
020 $a 9780542045035
035 $a (UMI)AAI3168415
035 $a AAI3168415
040 $a UMI $c UMI
100 1 $a Wu, Guohui. $3 1296091
245 1 0 $a Interaction between poloxamers and cell membranes.
300 $a 216 p.
500 $a Adviser: Ka Yee C. Lee.
500 $a Source: Dissertation Abstracts International, Volume: 66-03, Section: B, page: 1492.
502 $a Thesis (Ph.D.)--The University of Chicago, 2005.
520 $a Poloxamers, a family of PEO-PPO-PEO triblock copolymers, are known to help seal electroporated cell membranes. However, the interaction mechanism between the cell membrane and poloxamer is not well understood. Using both phospholipid monolayers and bilayers to mimic cell membranes, we have examined the lipid/poloxamer interaction between poloxamer and cell membranes.
520 $a X-ray reflectivity and grazing incidence x-ray diffraction measurements were performed on lipid monolayers mimicking the outer leaflet of the cell membrane. Our results show that at low nominal lipid density, P188 " corrals" lipid molecules by physically occupying the available area and phase separating from them, thus forcing the lipid molecules to pack tightly and restoring the membrane's barrier function. Upon compression to the bilayer equivalent pressure, P188 is squeezed out from the lipid monolayer, allowing a graceful exit of P188 when the membrane integrity is restored. Atomic force microscopy on the transferred thin film from air-water interface to a solid substrate reveals the accompanying morphological change and pinpoints the location of poloxamer in the lipid minolayer.
520 $a Isothermal titration calorimetry results show that poloxamer is only incorporated into fluid-phase lipid bilayer but not gel-phase ones, indicating that the lipid packing in different membrane structures plays a critical role in the lipid-poloxamer interaction. It is found that within the experimental time scale (200 min), the poloxamer is kinetically trapped in the outer leaflet of membrane, but can diffuse across the bilayer to reach the inner leaflet membrane with a longer time scale. The size distribution of fluid-phase liposomes decreases with the incorporation of poloxamer. Cryo-electron microscopy shows that the mixed lipid/poloxamer system transforms from spherical vesicles to bilayer disks when temperature is decreased through the bilayer main transition temperature. Atomic force microscopy further confirms the preferential adsorption of poloxamer onto defects in supported bilayer.
590 $a School code: 0330.
650 4 $a Biophysics, General. $3 1019105
650 4 $a Chemistry, Physical. $3 560527
690 $a 0494
690 $a 0786
710 2 0 $a The University of Chicago. $3 1017389
773 0 $t Dissertation Abstracts International $g 66-03B.
790 $a 0330
790 1 0 $a Lee, Ka Yee C., $e advisor
791 $a Ph.D.
792 $a 2005
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3168415