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The effect of extracellular osmolali...

Antolic, AnaMaria.

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The effect of extracellular osmolality on cell volume and resting skeletal muscle metabolism.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	The effect of extracellular osmolality on cell volume and resting skeletal muscle metabolism./
作者:	Antolic, AnaMaria.
面頁冊數:	138 p.
附註:	Source: Masters Abstracts International, Volume: 45-01, page: 0209.
Contained By:	Masters Abstracts International45-01.
標題:	Biology, Cell. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=MR17985
ISBN:	9780494179857

The effect of extracellular osmolality on cell volume and resting skeletal muscle metabolism.
Antolic, AnaMaria.

The effect of extracellular osmolality on cell volume and resting skeletal muscle metabolism. - 138 p.

Source: Masters Abstracts International, Volume: 45-01, page: 0209.

Thesis (M.Sc.)--Brock University (Canada), 2006.

The purpose of the current investigation was to establish an in-vitro skeletal muscle model to study acute alterations in resting skeletal muscle cell volume. Isolated, whole muscle (SOL and EDL) was dissected from Long Evans rats and incubated for 60 min in Sigma Medium-199 (resting tension (1g), bubbled with 95:5% O2:CO2, 30+/-2°C, and pH 7.4). Media osmolality was altered to simulate hypo-osmotic (190+/-10 Osm) (HYPO) or hyper-osmotic conditions (400+/-10 Osm) (HYPER) while an iso-osmotic condition (290+/-10 Osm) (CON) served as a control (n=17,19,17). Following incubation, relative muscle water content decreased with HYPER and increased with HYPO in both muscle types (p<0.05). The cross-sectional area of HYPO SOL type I and type It fibres increased (p<0.05) while the EDL type II fibre area decreased in HYPER and increased from HYPO exposure. Furthermore, HYPER exposure in both muscles lead to decreased ATP and phosphocreatine (p<0.05) and increased creatine and lactate (p<0.05) compared to CON. This isolated skeletal muscle model proved viable and demonstrated that altering extracellular osmolality could cause acute alterations in muscle water content and resting muscle metabolism.

ISBN: 9780494179857Subjects--Topical Terms:

1017686
Biology, Cell.

The effect of extracellular osmolality on cell volume and resting skeletal muscle metabolism.
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520 $a The purpose of the current investigation was to establish an in-vitro skeletal muscle model to study acute alterations in resting skeletal muscle cell volume. Isolated, whole muscle (SOL and EDL) was dissected from Long Evans rats and incubated for 60 min in Sigma Medium-199 (resting tension (1g), bubbled with 95:5% O2:CO2, 30+/-2°C, and pH 7.4). Media osmolality was altered to simulate hypo-osmotic (190+/-10 Osm) (HYPO) or hyper-osmotic conditions (400+/-10 Osm) (HYPER) while an iso-osmotic condition (290+/-10 Osm) (CON) served as a control (n=17,19,17). Following incubation, relative muscle water content decreased with HYPER and increased with HYPO in both muscle types (p<0.05). The cross-sectional area of HYPO SOL type I and type It fibres increased (p<0.05) while the EDL type II fibre area decreased in HYPER and increased from HYPO exposure. Furthermore, HYPER exposure in both muscles lead to decreased ATP and phosphocreatine (p<0.05) and increased creatine and lactate (p<0.05) compared to CON. This isolated skeletal muscle model proved viable and demonstrated that altering extracellular osmolality could cause acute alterations in muscle water content and resting muscle metabolism.
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