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Development of a cellular fiber spin...

Jones, Willie Franklin, Jr.

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Development of a cellular fiber spinning technology for regenerative medicine.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Development of a cellular fiber spinning technology for regenerative medicine./
作者:	Jones, Willie Franklin, Jr.
面頁冊數:	138 p.
附註:	Adviser: Karen J. L. Burg.
Contained By:	Masters Abstracts International45-02.
標題:	Engineering, Biomedical. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1439228
ISBN:	9780542958366

Development of a cellular fiber spinning technology for regenerative medicine.
Jones, Willie Franklin, Jr.

Development of a cellular fiber spinning technology for regenerative medicine. - 138 p.

Adviser: Karen J. L. Burg.

Thesis (M.S.)--Clemson University, 2006.

The prolonged success of cellular encapsulation in delivering insulin to treat Type I Diabetes has encouraged researchers to conceptualize ways in which cellular encapsulation can be implemented in an array of other clinical applications. This thesis investigated the development of a novel in-house textile hollow fiber spinning process in order to encapsulate cells. After development of the cellular encapsulation methods, a 21 day in vitro macroscopic evaluation was employed to confirm cellular viability and quantify the metabolic activity response of green fluorescent protein (GFP) labeled bovine mammary epithelial cells (MAC-Ts) that were either suspended in a low viscosity sodium alginate based solution, manually injected into the lumen of the hollow fiber (MI method), and later immobilized through a gelation process; or pre-mixed and co-extruded with a medium viscosity sodium alginate based solution (CO-X method), and immobilized inside the walls of the hollow fiber. No decrease in fluorescence was observed, and it was found that the CO-X and MI methods provided total lactic acid productions of 1.6 and 1.5 g/L glucose consumptions of 6.1 and 4.0 g/L, respectively, after 21 days of culturing. Histomorphological analyses revealed that the average cell area of MAC-Ts increased 25 and 88% after 21 days of encapsulation under the CO-X and MI methods, respectively. There was little to no evidence of cell clusters, and oval, cobblestone morphologies. Based on our findings, it is concluded that our novel in-house textile hollow fiber spinning process can be used to encapsulate cells using the CO-X and MI methods.

ISBN: 9780542958366Subjects--Topical Terms:

1017684
Engineering, Biomedical.

Development of a cellular fiber spinning technology for regenerative medicine.
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502 $a Thesis (M.S.)--Clemson University, 2006.
520 $a The prolonged success of cellular encapsulation in delivering insulin to treat Type I Diabetes has encouraged researchers to conceptualize ways in which cellular encapsulation can be implemented in an array of other clinical applications. This thesis investigated the development of a novel in-house textile hollow fiber spinning process in order to encapsulate cells. After development of the cellular encapsulation methods, a 21 day in vitro macroscopic evaluation was employed to confirm cellular viability and quantify the metabolic activity response of green fluorescent protein (GFP) labeled bovine mammary epithelial cells (MAC-Ts) that were either suspended in a low viscosity sodium alginate based solution, manually injected into the lumen of the hollow fiber (MI method), and later immobilized through a gelation process; or pre-mixed and co-extruded with a medium viscosity sodium alginate based solution (CO-X method), and immobilized inside the walls of the hollow fiber. No decrease in fluorescence was observed, and it was found that the CO-X and MI methods provided total lactic acid productions of 1.6 and 1.5 g/L glucose consumptions of 6.1 and 4.0 g/L, respectively, after 21 days of culturing. Histomorphological analyses revealed that the average cell area of MAC-Ts increased 25 and 88% after 21 days of encapsulation under the CO-X and MI methods, respectively. There was little to no evidence of cell clusters, and oval, cobblestone morphologies. Based on our findings, it is concluded that our novel in-house textile hollow fiber spinning process can be used to encapsulate cells using the CO-X and MI methods.
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