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Development of a single nucleotide p...

Zhang, Hai.

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Development of a single nucleotide polymorphism-based electronic DNA microarray technique for the detection and species differentiation of viable Campylobacter.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Development of a single nucleotide polymorphism-based electronic DNA microarray technique for the detection and species differentiation of viable Campylobacter./
作者:	Zhang, Hai.
面頁冊數:	135 p.
附註:	Source: Masters Abstracts International, Volume: 44-06, page: 2784.
Contained By:	Masters Abstracts International44-06.
標題:	Biology, Microbiology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=MR13919
ISBN:	9780494139196

Development of a single nucleotide polymorphism-based electronic DNA microarray technique for the detection and species differentiation of viable Campylobacter.
Zhang, Hai.

Development of a single nucleotide polymorphism-based electronic DNA microarray technique for the detection and species differentiation of viable Campylobacter. - 135 p.

Source: Masters Abstracts International, Volume: 44-06, page: 2784.

Thesis (M.Sc.)--University of Alberta (Canada), 2006.

Campylobacter jejuni, C. coli, and C. lari are the three major food-borne pathogenic Campylobacter species that cause the most frequent occurrences of acute bacterial gastroenteritis around the world. This thesis presents the successful development of a reverse transcription-polymerase chain reaction (RT-PCR) electronic DNA microarray approach for the simultaneous detection and species differentiation of viable Campylobacter. The mRNA of the 60-kDa heat shock protein gene ( hsp60) was used as the viability marker, and two closely located single nucleotide polymorphisms (SNPs) within this gene were chosen as the species marker. A 200-bp fragment amplified from the hsp60 mRNA by RT-PCR was detected with species-specific fluorescently-labeled reporters using an electronic DNA microarray technique.

ISBN: 9780494139196Subjects--Topical Terms:

1017734
Biology, Microbiology.

Development of a single nucleotide polymorphism-based electronic DNA microarray technique for the detection and species differentiation of viable Campylobacter.
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520 $a Campylobacter jejuni, C. coli, and C. lari are the three major food-borne pathogenic Campylobacter species that cause the most frequent occurrences of acute bacterial gastroenteritis around the world. This thesis presents the successful development of a reverse transcription-polymerase chain reaction (RT-PCR) electronic DNA microarray approach for the simultaneous detection and species differentiation of viable Campylobacter. The mRNA of the 60-kDa heat shock protein gene ( hsp60) was used as the viability marker, and two closely located single nucleotide polymorphisms (SNPs) within this gene were chosen as the species marker. A 200-bp fragment amplified from the hsp60 mRNA by RT-PCR was detected with species-specific fluorescently-labeled reporters using an electronic DNA microarray technique.
520 $a This technique can detect as few as two viable Campylobacter cells, and will not detect dead cells. The evaluation of 14 blind Campylobacter samples showed 100% agreement with their identities, demonstrating its high specificity. This technique was preliminarily applied to six authentic chicken samples as well.
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