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Biophotonic studies of mammalian cel...

Sun, Yinghua.

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Biophotonic studies of mammalian cells with nanosecond pulsed power and fluorescent quantum dots.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Biophotonic studies of mammalian cells with nanosecond pulsed power and fluorescent quantum dots./
作者:	Sun, Yinghua.
面頁冊數:	113 p.
附註:	Adviser: Martin A. Gundersen.
Contained By:	Dissertation Abstracts International67-10B.
標題:	Biophysics, Medical. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3237737
ISBN:	9780542924033

Biophotonic studies of mammalian cells with nanosecond pulsed power and fluorescent quantum dots.
Sun, Yinghua.

Biophotonic studies of mammalian cells with nanosecond pulsed power and fluorescent quantum dots. - 113 p.

Adviser: Martin A. Gundersen.

Thesis (Ph.D.)--University of Southern California, 2006.

Electroperturbation, the non-invasive manipulation of intracellular organelles and functions of biological cells achieved by nanosecond pulsed electric fields, is a potential technique for cancer therapy and biophysical research. This work requires surmounting technological challenges including the flexible generation of nanosecond, megavolt-per-meter electric fields and the assembly of visualization systems for dynamic imaging of cellular structures and processes during pulse exposure. We developed an integrated fluorescence microscopy imaging system for investigations of electroperturbation of mammalian cells. Integrating a solid-state high-voltage pulser, an innovative MEMS electrode chamber, and a sensitive digital imaging system with an epi-fluorescence microscope, this system allowed in vitro study of apoptosis and dynamic responses of mammalian cells exposed to nanoelectropulses. Calcium bursts, phosphatidylserine polarized externalization, nuclear perturbation, dynamic membrane permeabilization, and cardiomyocyte stimulation induced by nanoelectropulses were recorded and investigated in this system. We studied electroperturbation in a number of cell lines and primary cells including human Jurkat lymphocytes, multiple myeloma RPMI 8226, ovarian cancer SKOV-3, breast cancer MCF7, pancreatic cancer AsPc-1, bovine adrenal chromaffin cells, and rabbit cardiomyocytes. These results point to promising applications for nanoelectropulse technology in cancer treatment and cell stimulation.

ISBN: 9780542924033Subjects--Topical Terms:

1017681
Biophysics, Medical.

Biophotonic studies of mammalian cells with nanosecond pulsed power and fluorescent quantum dots.
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245 1 0 $a Biophotonic studies of mammalian cells with nanosecond pulsed power and fluorescent quantum dots.
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500 $a Source: Dissertation Abstracts International, Volume: 67-10, Section: B, page: 5890.
502 $a Thesis (Ph.D.)--University of Southern California, 2006.
520 $a Electroperturbation, the non-invasive manipulation of intracellular organelles and functions of biological cells achieved by nanosecond pulsed electric fields, is a potential technique for cancer therapy and biophysical research. This work requires surmounting technological challenges including the flexible generation of nanosecond, megavolt-per-meter electric fields and the assembly of visualization systems for dynamic imaging of cellular structures and processes during pulse exposure. We developed an integrated fluorescence microscopy imaging system for investigations of electroperturbation of mammalian cells. Integrating a solid-state high-voltage pulser, an innovative MEMS electrode chamber, and a sensitive digital imaging system with an epi-fluorescence microscope, this system allowed in vitro study of apoptosis and dynamic responses of mammalian cells exposed to nanoelectropulses. Calcium bursts, phosphatidylserine polarized externalization, nuclear perturbation, dynamic membrane permeabilization, and cardiomyocyte stimulation induced by nanoelectropulses were recorded and investigated in this system. We studied electroperturbation in a number of cell lines and primary cells including human Jurkat lymphocytes, multiple myeloma RPMI 8226, ovarian cancer SKOV-3, breast cancer MCF7, pancreatic cancer AsPc-1, bovine adrenal chromaffin cells, and rabbit cardiomyocytes. These results point to promising applications for nanoelectropulse technology in cancer treatment and cell stimulation.
520 $a In the second part of the thesis, we explored bioapplications of colloidal quantum dots (QDs), which are promising fluorescence probes but still face challenges including intracellular targeting and the photostability in biological systems. Electroporation and endocytosis were studied as means to transfer QDs into mammalian cells, and the photophysical properties of QDs within living cells were investigated. The efficient delivery of QDs by electroporation was achieved after optimizing multiple factors affecting QD transportation, such as electric field, pulse width, pulsing medium, cell type, and particle size. Selective endocytosis QDs by mammalian cells was obtained by using mercaptoacetic-acid-capped (MAA) CdSe/ZnSe/ZnS QDs synthesized in our lab. Studies on interactions between QDs and biological systems revealed the pH-dependence and a significant photoinduced luminance enhancement (photoactivation) of MAA QD fluorescence. We found that electroporation is an appropriate technique for cellular QD delivery and that MAA QD is a promising candidate for intracellular environmental sensors.
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