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Genomic analysis of Drosophila trach...

Chao, Elizabeth Danhwa.

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Genomic analysis of Drosophila tracheal organogenesis: Identification of a transcriptional target of Trachealess mediating cell adhesion and motility.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Genomic analysis of Drosophila tracheal organogenesis: Identification of a transcriptional target of Trachealess mediating cell adhesion and motility./
作者:	Chao, Elizabeth Danhwa.
面頁冊數:	127 p.
附註:	Adviser: Mark A. Krasnow.
Contained By:	Dissertation Abstracts International68-06B.
標題:	Biology, Cell. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3267478
ISBN:	9780549061632

Genomic analysis of Drosophila tracheal organogenesis: Identification of a transcriptional target of Trachealess mediating cell adhesion and motility.
Chao, Elizabeth Danhwa.

Genomic analysis of Drosophila tracheal organogenesis: Identification of a transcriptional target of Trachealess mediating cell adhesion and motility. - 127 p.

Adviser: Mark A. Krasnow.

Thesis (Ph.D.)--Stanford University, 2007.

The extraordinary developmental process of organogenesis, from the differentiation of multi-potential progenitors to the construction of a three-dimensional, functionally specialized organ, requires exquisite molecular and cellular coordination. Yet very little is known about the events culminating in the creation of an organ, particularly in vertebrate organisms, because they are less amenable to genetic and embryological analysis. The Drosophila airway (tracheal system), with its simple, yet exquisite branching pattern and genetic tractability, has emerged as an excellent model to study organogenesis. Functioning at the top of the genetic regulatory hierarchy governing the organogenesis of the tracheal system is a gene encoding the basic-helix-loop-helix-PAS domain transcription factor, Trachealess (Trh). In an effort to illuminate the gene expression program required for tracheal organogenesis, we performed a genomic screen using full genome Drosophila cDNA microarrays and complemented the results with an analysis of 5,270 embryonic in situ expression patterns. Whole-genome expression profiles of wildtype versus trachealess mutant embryos revealed that 75% of genes expressed selectively in the early tracheal system rely on Trh for their expression. In addition, these genes were 25-fold enriched for conserved clusters of Trh binding motifs, suggesting that they are likely to be under the direct control of Trh. Consistent with this observation, we identified tracheal enhancers proximal to the promoters of two candidate Trh target genes, CG3777 and polychaetoid (pyd). Subsequent construction of in vivo enhancer reporters and mutagenesis of the Trh binding sites within each enhancer demonstrated that both CG3777 and pyd are in fact direct targets of Trh. Further characterization of pyd, a membrane associated guanylate kinase (MAGUK) with homology to mammalian Zonula occludens-1 (ZO-1), suggests an essential role in a novel, non-canonical Notch-mediated cell migration and adhesion process during early tracheal development. The results of these studies constitute the first comprehensive analysis of the gene expression program governing Drosophila tracheal system organogenesis.

ISBN: 9780549061632Subjects--Topical Terms:

1017686
Biology, Cell.

Genomic analysis of Drosophila tracheal organogenesis: Identification of a transcriptional target of Trachealess mediating cell adhesion and motility.
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