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Development of bio-photonic sensor b...

Kim, Chan Kyu.

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Development of bio-photonic sensor based on laser-induced fluorescence.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Development of bio-photonic sensor based on laser-induced fluorescence./
Author:	Kim, Chan Kyu.
Description:	135 p.
Notes:	Adviser: Jagdish P. Singh.
Contained By:	Dissertation Abstracts International69-01B.
Subject:	Biophysics, General. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3296215
ISBN:	9780549410331

Development of bio-photonic sensor based on laser-induced fluorescence.
Kim, Chan Kyu.

Development of bio-photonic sensor based on laser-induced fluorescence. - 135 p.

Adviser: Jagdish P. Singh.

Thesis (Ph.D.)--Mississippi State University, 2007.

Key words. Laser induced fluorescence, human breast cancer cell lines, Azotobacter vinelandii, DNA, gold nanoparticles.

ISBN: 9780549410331Subjects--Topical Terms:

1019105
Biophysics, General.

Development of bio-photonic sensor based on laser-induced fluorescence.
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020 $a 9780549410331
035 $a (UMI)AAI3296215
035 $a AAI3296215
040 $a UMI $c UMI
100 1 $a Kim, Chan Kyu. $3 1285550
245 1 0 $a Development of bio-photonic sensor based on laser-induced fluorescence.
300 $a 135 p.
500 $a Adviser: Jagdish P. Singh.
500 $a Source: Dissertation Abstracts International, Volume: 69-01, Section: B, page: 0387.
502 $a Thesis (Ph.D.)--Mississippi State University, 2007.
520 $a Key words. Laser induced fluorescence, human breast cancer cell lines, Azotobacter vinelandii, DNA, gold nanoparticles.
520 $a Laser-induced fluorescence (LIF) has been shown to be potentially useful for identifying microorganisms in real time. It is a selective and sensitive technique because the excitation is performed at one wavelength while the emission is monitored at longer wavelengths so that background from the excitation source can be eliminated. This specialized optical property of LIF can be applied to development of an optical sensor capable of quickly, non-invasively, and quantitatively probing complex biochemical transformations in microorganisms. Various bio-photonic optical fiber sensors based on laser-induced fluorescence (LIF) spectroscopy were developed as diagnostic tools for microorganisms. In the first phase, the enhancement of the sensitivity and selectivity of the optical sensor system focused on diagnosis of human breast cancer cell lines and Azotobacter vinelandii (an aerobic soil-dwelling organism). Auto-fluorescence spectra from human breast cancer cell lines and Azotobacter vinelandii corresponding to different growth environments were investigated. Then, the study has expanded to include the use of gold nanoparticles for specific DNA detection. The use of gold nanoparticles opens a door into construction of a compact, highly specific, inexpensive and user-friendly optical fiber senor for specific DNA detection. An optical fiber laser-induced fluorescence (LIF) sensor based has been developed to detect single-strand (ss) DNA hybridization at the femtomolar level. Effects of various experimental parameters and configuration were investigated in order to optimize sensor performance and miniaturize sensor size.
590 $a School code: 0132.
650 4 $a Biophysics, General. $3 1019105
650 4 $a Physics, Molecular. $3 1018648
650 4 $a Physics, Optics. $3 1018756
690 $a 0609
690 $a 0752
690 $a 0786
710 2 $a Mississippi State University. $b Physics and Astronomy. $3 1285551
773 0 $t Dissertation Abstracts International $g 69-01B.
790 $a 0132
790 1 0 $a Singh, Jagdish P., $e advisor
791 $a Ph.D.
792 $a 2007
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3296215