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Study of the coupling between transc...

Devanney, Sean C.

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Study of the coupling between transcription and mRNA processing utilizing a novel Bcl-x mini-gene.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Study of the coupling between transcription and mRNA processing utilizing a novel Bcl-x mini-gene./
作者:	Devanney, Sean C.
面頁冊數:	55 p.
附註:	Adviser: Massimo Caputi.
Contained By:	Masters Abstracts International46-02.
標題:	Biology, Cell. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1449181
ISBN:	9780549286547

Study of the coupling between transcription and mRNA processing utilizing a novel Bcl-x mini-gene.
Devanney, Sean C.

Study of the coupling between transcription and mRNA processing utilizing a novel Bcl-x mini-gene. - 55 p.

Adviser: Massimo Caputi.

Thesis (M.S.)--Florida Atlantic University, 2007.

The Bcl family of genes are fundamental to the apoptotic mechanism. Bcl-x a member of this family, is alternatively spliced to create two main isoforms a long (Bcl-xL) and a short (Bcl-xS) variant. The long form exhibits anti-apoptotic activity, while the short form favors apoptosis. The proper balance of expression of these two isoforms is crucial for several developmental processes such as thymic selection and neural reshaping. A number of cancer types have been shown to over-express the long form, thereby granting them some protection from apoptosis. To study the transcriptional and post-transcriptional mechanisms regulating gene expression, the Bcl-x gene has been utilized. A complex mini-gene construct has been create in order to monitor the effects that promoter sequences, 5'UTR and 3'UTR's have on mRNA splicing, RNA export, stability and translation. Abundant evidence exists indicating that RNA processing events such as transcription, splicing and export are coupled, yet the mechanisms and factors involved in regulating these processes are poorly understood. The mini-gene is identical to the endogenous gene with the exception of a deletion to the 50Kb intron and the addition of a tag to differentiate the mini-gene product from the endogenous mRNA and protein. This novel system allows for the study of transcriptional and post-transcriptional mechanisms regulating gene expression from RNA biogenesis on to the protein level.

ISBN: 9780549286547Subjects--Topical Terms:

1017686
Biology, Cell.

Study of the coupling between transcription and mRNA processing utilizing a novel Bcl-x mini-gene.
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