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Positional cloning of Gpc-B1, a whea...

Uauy, Cristobal.

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Positional cloning of Gpc-B1, a wheat quantitative trait loci affecting senescence and with pleiotropic effects on grain protein, zinc and iron concentration.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Positional cloning of Gpc-B1, a wheat quantitative trait loci affecting senescence and with pleiotropic effects on grain protein, zinc and iron concentration./
作者:	Uauy, Cristobal.
面頁冊數:	70 p.
附註:	Adviser: Jorge Dubcousky.
Contained By:	Dissertation Abstracts International68-09B.
標題:	Agriculture, Plant Culture. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3280654
ISBN:	9780549219897

Positional cloning of Gpc-B1, a wheat quantitative trait loci affecting senescence and with pleiotropic effects on grain protein, zinc and iron concentration.
Uauy, Cristobal.

Positional cloning of Gpc-B1, a wheat quantitative trait loci affecting senescence and with pleiotropic effects on grain protein, zinc and iron concentration. - 70 p.

Adviser: Jorge Dubcousky.

Thesis (Ph.D.)--University of California, Davis, 2007.

Enhancing the nutritional value of food crops is a sensible strategy for improving human health and nutrition. Despite its importance, efforts to increase wheat grain protein content (GPC) and micronutrient levels have been hindered by their large environmental dependency and complex inheritance. A potential source for nutritional improvement was detected in wild emmer wheat (Triticum turgidum ssp. dicoccoides), where a QTL for GPC (Gpc-B1) was shown to confer consistent GPC increases when introgressed into tetraploid and hexaploid wheat varieties (average 14 g*kg-1 (&sim;10-15%)).

ISBN: 9780549219897Subjects--Topical Terms:

1018669
Agriculture, Plant Culture.

Positional cloning of Gpc-B1, a wheat quantitative trait loci affecting senescence and with pleiotropic effects on grain protein, zinc and iron concentration.
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245 1 0 $a Positional cloning of Gpc-B1, a wheat quantitative trait loci affecting senescence and with pleiotropic effects on grain protein, zinc and iron concentration.
300 $a 70 p.
500 $a Adviser: Jorge Dubcousky.
500 $a Source: Dissertation Abstracts International, Volume: 68-09, Section: B, page: 5716.
502 $a Thesis (Ph.D.)--University of California, Davis, 2007.
520 $a Enhancing the nutritional value of food crops is a sensible strategy for improving human health and nutrition. Despite its importance, efforts to increase wheat grain protein content (GPC) and micronutrient levels have been hindered by their large environmental dependency and complex inheritance. A potential source for nutritional improvement was detected in wild emmer wheat (Triticum turgidum ssp. dicoccoides), where a QTL for GPC (Gpc-B1) was shown to confer consistent GPC increases when introgressed into tetraploid and hexaploid wheat varieties (average 14 g*kg-1 (&sim;10-15%)).
520 $a To isolate the gene underlying Gpc-B1, we developed a 250-kb physical map of the Gpc-B1 region using a tetraploid wheat bacterial artificial chromosome library. Using a high-resolution genetic map based on 9000 gametes, we delimited Gpc-B1 to 7.4-kb. This region included a single gene encoding a NAC transcription factor. Modern cultivated wheat varieties carry either a 1-bp insertion generating a frameshift mutation resulting in a non-functional transcription factor, or a deletion of the gene, whereas the ancestral wild emmer allele encodes a functional protein.
520 $a We showed that the 7.4 kb segment containing the high GPC allele accelerates senescence (3-4 days) and increases N, Zn and Fe remobilization from leaves to developing grains (10-15%). To validate this gene as Gpc-B1, we reduced the RNA transcript levels of the multiple homologous copies in hexaploid wheat by RNA interference. Two transgenic events generated the expected phenotype: senescence was delayed by over 3 weeks and grain protein, Zn, and Fe content was decreased by over 30%. The absence of the functional Gpc-B1 allele in modern germplasm suggests a broad potential impact of the functional allele in cultivated durum and bread wheat varieties.
520 $a The cloning of Gpc-B1 illustrates the feasibility of map-based cloning of QTL in the large genomes of polyploid wheat and provides an entry point to characterize the complex network of genes that regulates both senescence and nutrient remobilization in an agronomically important species. More importantly, it provides a direct link between these processes. This information may contribute to a more efficient manipulation of these processes in crop species and will potentially translate into better nutrition for the world's malnourished.
590 $a School code: 0029.
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650 4 $a Biology, Genetics. $3 1017730
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690 $a 0479
710 2 $a University of California, Davis. $3 1018682
773 0 $t Dissertation Abstracts International $g 68-09B.
790 $a 0029
790 1 0 $a Dubcousky, Jorge, $e advisor
791 $a Ph.D.
792 $a 2007
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