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Phase 2 enzyme-inducing agents from ...

Bolling, Bradley W.

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Phase 2 enzyme-inducing agents from soybean (Glycine max).

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Phase 2 enzyme-inducing agents from soybean (Glycine max)./
Author:	Bolling, Bradley W.
Description:	187 p.
Notes:	Adviser: Kirk L. Parkin.
Contained By:	Dissertation Abstracts International68-12B.
Subject:	Agriculture, Food Science and Technology. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3294029
ISBN:	9780549383246

Phase 2 enzyme-inducing agents from soybean (Glycine max).
Bolling, Bradley W.

Phase 2 enzyme-inducing agents from soybean (Glycine max). - 187 p.

Adviser: Kirk L. Parkin.

Thesis (Ph.D.)--The University of Wisconsin - Madison, 2007.

Soy flour and protein can induce the cancer chemopreventive phase 2 detoxification enzymes when fed to rats. However, the phytochemicals responsible for this effect have not been previously identified. The objectives of this work were to isolate, identify, and characterize phase 2 enzyme-inducing agents from soybean using cellular bioassay-directed isolation and to evaluate the efficacy of inducing agent(s) from soy in vivo.

ISBN: 9780549383246Subjects--Topical Terms:

1017813
Agriculture, Food Science and Technology.

Phase 2 enzyme-inducing agents from soybean (Glycine max).
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020 $a 9780549383246
035 $a (UMI)AAI3294029
035 $a AAI3294029
040 $a UMI $c UMI
100 1 $a Bolling, Bradley W. $3 1272243
245 1 0 $a Phase 2 enzyme-inducing agents from soybean (Glycine max).
300 $a 187 p.
500 $a Adviser: Kirk L. Parkin.
500 $a Source: Dissertation Abstracts International, Volume: 68-12, Section: B, page: 7722.
502 $a Thesis (Ph.D.)--The University of Wisconsin - Madison, 2007.
520 $a Soy flour and protein can induce the cancer chemopreventive phase 2 detoxification enzymes when fed to rats. However, the phytochemicals responsible for this effect have not been previously identified. The objectives of this work were to isolate, identify, and characterize phase 2 enzyme-inducing agents from soybean using cellular bioassay-directed isolation and to evaluate the efficacy of inducing agent(s) from soy in vivo.
520 $a Soy flour extracts were fractionated and screened for quinone reductase (QR) induction in murine hepa 1c1e7 cells, a biomarker for phase 2 enzyme induction. Sequential Soxhlet solvent extraction of soy flour with ethyl acetate and ethanol yielded a fraction enriched in phospholipids that doubled QR specific activity (CD-value, concentration required to double) at 460 mug/ml. Phospholipid depletion and solvent partitioning were utilized to further enrich the inducing activity of the ethanol isolate to give a CD value of 80 mug/mL. This fraction was resolved by reverse phase chromatography to produce 13 fractions. Individual isoflavone glycosides were isolated as QR inducers, but were not as potent as their aglycone forms. Fractions 11-13 were the most potent with CD values of 10, 6, and 7 mug/ml, respectively.
520 $a To isolate components of fractions 11-13 more efficiently, normal phase chromatography was used to obtain 8 fractions. Silica fractions 1-3 were the most active, with CD-values ranging from 1.9-8.2 mug/mL. Among these fractions were monoacylglyerols, benzofurans, p-hydroxybenzoic acid ethyl ester, 4-ethoxy cinnamic acid, and ferulic acid ethyl ester (FAEE). FAEE was found to be the most potent of the isolates with a CD value of 3.4 muM.
520 $a Ferulic acid (FA), a known phase 2 enzyme inducer, and FAEE were fed to rats to test for the ability to up-regulate cancer chemoproteetive enzymes. Liver, kidney, intestine, colon, lung, and brain tissues were collected and analyzed for QR, glutathione-S-transferase (GST), thioredoxin reductase, and cytochrome P450 specific activities. FA and FAEE induced cytosolic QR and GST specific activities and improved glutathione redox status in the kidney and colon. FARE also induced QR in the intestine.
590 $a School code: 0262.
650 4 $a Agriculture, Food Science and Technology. $3 1017813
650 4 $a Chemistry, Biochemistry. $3 1017722
690 $a 0359
690 $a 0487
710 2 $a The University of Wisconsin - Madison. $3 626640
773 0 $t Dissertation Abstracts International $g 68-12B.
790 $a 0262
790 1 0 $a Parkin, Kirk L., $e advisor
791 $a Ph.D.
792 $a 2007
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3294029