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Genomic instability of infertile hum...

Abdel Megid, Wael M.

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Genomic instability of infertile human spermatozoa and somatic cells exposed to free radicals.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Genomic instability of infertile human spermatozoa and somatic cells exposed to free radicals./
作者:	Abdel Megid, Wael M.
面頁冊數:	176 p.
附註:	Advisers: Neal First; Marijo Kent-First.
Contained By:	Dissertation Abstracts International68-08B.
標題:	Biology, Cell. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3278827
ISBN:	9780549195276

Genomic instability of infertile human spermatozoa and somatic cells exposed to free radicals.
Abdel Megid, Wael M.

Genomic instability of infertile human spermatozoa and somatic cells exposed to free radicals. - 176 p.

Advisers: Neal First; Marijo Kent-First.

Thesis (Ph.D.)--The University of Wisconsin - Madison, 2007.

Two general types of DNA damage characterize the male germline. The first type is damage leading to errors and gene mutations that occur during the course of cell division. The second type is damage leading to errors in mismatch repair (MMR) or recombination errors and DNA and chromatin fragmentation, specifically DNA strand breaks. The thesis of the following dissertation states that, genomic instability in germ cells of men with partial or complete spermatogenic arrest is due to oxidative stress, and these infertile men may be at an associated increased risk of developing testicular cancer when damage from free radicals override mismatch repair (MMR) or other DNA repair mechanisms. To test this hypothesis I measured instability utilizing markers of variable repeat motifs in sperm of infertile men with partial or complete spermatogenic arrest compared to sperm in fertile men and the associated somatic cells.

ISBN: 9780549195276Subjects--Topical Terms:

1017686
Biology, Cell.

Genomic instability of infertile human spermatozoa and somatic cells exposed to free radicals.
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245 1 0 $a Genomic instability of infertile human spermatozoa and somatic cells exposed to free radicals.
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500 $a Source: Dissertation Abstracts International, Volume: 68-08, Section: B, page: 5070.
502 $a Thesis (Ph.D.)--The University of Wisconsin - Madison, 2007.
520 $a Two general types of DNA damage characterize the male germline. The first type is damage leading to errors and gene mutations that occur during the course of cell division. The second type is damage leading to errors in mismatch repair (MMR) or recombination errors and DNA and chromatin fragmentation, specifically DNA strand breaks. The thesis of the following dissertation states that, genomic instability in germ cells of men with partial or complete spermatogenic arrest is due to oxidative stress, and these infertile men may be at an associated increased risk of developing testicular cancer when damage from free radicals override mismatch repair (MMR) or other DNA repair mechanisms. To test this hypothesis I measured instability utilizing markers of variable repeat motifs in sperm of infertile men with partial or complete spermatogenic arrest compared to sperm in fertile men and the associated somatic cells.
520 $a My results show that overall microsatellite instability occurs significantly more in the ejaculated sperm of infertile men than in normally fertile men. More specifically, men with partial or complete meiotic arrest and men with partial or complete meiotic arrest that developed a germline tumor exhibited a significantly higher level of germline specific MSI than the control group. Significantly higher levels of MSI frequency are seen in men with partial or complete spermatogenic arrest and also some of these men developed germ cell tumors. There is a highly significant and direct correlation between % MSI and the % DFI that is measured by sperm chromatin structure assay for normozoospermic and oligozoospermic men.
520 $a In conclusion, my study established the high sensitivity of the Y-STR locus and extended mononucleotide repeat for MSI analysis and demonstrates that the majority of germ cells obtained from infertile men show some degree of low level microsattelite instabilities which were also instable in testicular cancer.
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