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Development of a direct-fed microbia...

Kim, Seon-Woo.

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Development of a direct-fed microbial for beef cattle.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Development of a direct-fed microbial for beef cattle./
作者:	Kim, Seon-Woo.
面頁冊數:	278 p.
附註:	Adviser: Steven R. Rust.
Contained By:	Dissertation Abstracts International69-01B.
標題:	Agriculture, Animal Culture and Nutrition. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3298062
ISBN:	9780549414957

Development of a direct-fed microbial for beef cattle.
Kim, Seon-Woo.

Development of a direct-fed microbial for beef cattle. - 278 p.

Adviser: Steven R. Rust.

Thesis (Ph.D.)--Michigan State University, 2007.

To minimize ruminal lactic acidosis in beef cattle, the development of a direct-fed microbial using lactic acid-utilizing bacteria was conducted. This study included an aerobic enrichment of rumen contents in a lactic acid medium, isolation of aero-tolerant lactic acid-utilizing bacteria, and in vitro and in vivo animal trials to evaluate isolates' effects on lactic acid utilization in the rumen.

ISBN: 9780549414957Subjects--Topical Terms:

1017857
Agriculture, Animal Culture and Nutrition.

Development of a direct-fed microbial for beef cattle.
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100 1 $a Kim, Seon-Woo. $3 1271291
245 1 0 $a Development of a direct-fed microbial for beef cattle.
300 $a 278 p.
500 $a Adviser: Steven R. Rust.
500 $a Source: Dissertation Abstracts International, Volume: 69-01, Section: B, page: 0005.
502 $a Thesis (Ph.D.)--Michigan State University, 2007.
520 $a To minimize ruminal lactic acidosis in beef cattle, the development of a direct-fed microbial using lactic acid-utilizing bacteria was conducted. This study included an aerobic enrichment of rumen contents in a lactic acid medium, isolation of aero-tolerant lactic acid-utilizing bacteria, and in vitro and in vivo animal trials to evaluate isolates' effects on lactic acid utilization in the rumen.
520 $a Aerobically enriched rumen microorganisms in a lactic acid medium decreased lactic acid production in the in vitro ruminal fermentation more efficiently than anaerobic enrichment. Aero-tolerant lactic acid-utilizing microorganisms were isolated from rumen contents enriched in an aerobic lactic acid media, and identified and designated to Megasphaera elsdenii (RK02) and Enterococcus faecium (RK03). The combination of these microorganisms grew in aerobic conditions. In vitro fermentations of ground corn or readily fermentable carbohydrate using rumen fluids collected from concentrate- or hay-diet adapted cows were conducted and the addition of RK02 and RK03 into the fermentation systems decreased lactic acid concentration, increased butyric and valeric acids at the expense of acetic and propionic acids, and increased pH. However, in the study of eight steers fed a high concentrate diet, RK02 and RK03 did not change the fermentation characteristics. The combination of RK02 and RK03 tended to increase butyric acid and tended to decrease propionic acid concentration. When eight steers were challenged with rapidly fermentable substrate (finely ground wheat), the combination of RK02 and RK03 facilitated the ruminal fermentation that resulted in greater concentration of total volatile fatty acid (VFA). This microbial treatment decreased partial pressure of carbon dioxide and increased partial pressure of oxygen in the blood of steers challenged with acidosis. DFM tended to have higher blood pH. Further study will he required to determine the appropriate dose, microbial mixture, and timing of administration of DFM to prevent acidotic conditions in the rumen.
520 $a In another animal performance study, Propionibacterium acidipropionici, strain DH42 was used as a DFM. DH42 did not establish a niche in the rumen of the steer; therefore DH42 was added daily as a top dress to the diet for a 123 d study. Between d 56 and 111, DH42 tended to decrease dry matter intake (DMI), but over the entire trial, performance was similar to the control. At the end of trial, cattle were harvested, and carcass yield grade was improved by DH42, but quality grade was similar. DH42 was active in the rumen and further research with other strains and dosages are warranted. Further isolation of lactic acid-utilizing bacteria using an aerobic enrichment may expand the choice of DFM candidates which can be used in the development of DFM.
590 $a School code: 0128.
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710 2 $a Michigan State University. $3 676168
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790 1 0 $a Rust, Steven R., $e advisor
791 $a Ph.D.
792 $a 2007
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