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Molecular characterization of heavy ...

Siripornadulsil, Surasak.

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Molecular characterization of heavy metal metabolism in transgenic microalgae (Chlamydomonas reinhardtii).

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Molecular characterization of heavy metal metabolism in transgenic microalgae (Chlamydomonas reinhardtii)./
作者:	Siripornadulsil, Surasak.
面頁冊數:	275 p.
附註:	Adviser: Richard T. Sayre.
Contained By:	Dissertation Abstracts International63-07B.
標題:	Biology, Microbiology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3059324
ISBN:	9780493748696

Molecular characterization of heavy metal metabolism in transgenic microalgae (Chlamydomonas reinhardtii).
Siripornadulsil, Surasak.

Molecular characterization of heavy metal metabolism in transgenic microalgae (Chlamydomonas reinhardtii). - 275 p.

Adviser: Richard T. Sayre.

Thesis (Ph.D.)--The Ohio State University, 2002.

On a global basis, trace-metal pollution is one of the most pervasive environmental problems because of the toxicity of their elemental form. In this work, we report the engineering of Chlamydomonas reinhardtii for uses in heavy metal bioremediation. The construction and successful application of the a universal vector (pSSCR7, 4.3 kbp) used for nuclear gene expression in C. reinhardtii is reported.

ISBN: 9780493748696Subjects--Topical Terms:

1017734
Biology, Microbiology.

Molecular characterization of heavy metal metabolism in transgenic microalgae (Chlamydomonas reinhardtii).
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100 1 $a Siripornadulsil, Surasak. $3 1270233
245 1 0 $a Molecular characterization of heavy metal metabolism in transgenic microalgae (Chlamydomonas reinhardtii).
300 $a 275 p.
500 $a Adviser: Richard T. Sayre.
500 $a Source: Dissertation Abstracts International, Volume: 63-07, Section: B, page: 3192.
502 $a Thesis (Ph.D.)--The Ohio State University, 2002.
520 $a On a global basis, trace-metal pollution is one of the most pervasive environmental problems because of the toxicity of their elemental form. In this work, we report the engineering of Chlamydomonas reinhardtii for uses in heavy metal bioremediation. The construction and successful application of the a universal vector (pSSCR7, 4.3 kbp) used for nuclear gene expression in C. reinhardtii is reported.
520 $a Metallothionein-II (MT) is a low molecular weight (&sim;61 to 62 amono acids), cysteine-rich two domain, metal-binding protein. The beta-domain preferentially binds monovalent metals and the beta-domain, divalent. We have constructed a fusion gene between a chicken MT-II gene and a Chlamydomonas plasma membrane protein-encoding gene for extracellular cadmium binding and protein stabilization. To increase the specificity and the binding capacity of transformants we have constructed membrane fusions of the alpha- and/or beta-domain of MT-II as well as a series of anchored polymers (1--5) of the MT-II domains separately. We show that these transgenic algae have substantially enhanced tolerance to toxic Cd concentrations (100 muM). In addition, the Cd binding capacity increased 2--5 fold relative to wild-type cells when grown in the presence of 50 muM Cd. Sensitivity to EDTA removal of the metal ions and EXAFS studies are consistent with coordination of the metal ions by the MT-II cysteinyl sulfides.
520 $a Free proline has been shown to play an important role in ameliorating environmental stress in plants and microorganisms, including heavy metal stress. We have expressed pyrroline-5-carboxylate synthase (Vigna PSCS from mothbean), the rate limiting step to proline synthesis, in C. reinhardtii. We show that transformants expressing the Vigna PSCS gene have 80% higher free-proline levels than wild-type cells, tolerate toxic Cd concentrations (100 muM), and bind four-fold more cadmium than wild-type cells grown in the presence of 50 muM Cd. The Cd-K edge extended X-ray absorption fine structure (EXAFS) studies reveals a predominantly tetrahedral coordination of cadmium by sulfur compared to a 2 sulfur/2 oxygen tetrahedral coordination in wild-type cells. Our results show that increases in free proline reduces free radical damage, maintains a more reducing environment and results in elevated glutathione levels in the transgenic cells.
590 $a School code: 0168.
650 4 $a Biology, Microbiology. $3 1017734
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Biophysics, General. $3 1019105
650 4 $a Engineering, Environmental. $3 783782
690 $a 0307
690 $a 0410
690 $a 0775
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710 2 $a The Ohio State University. $3 718944
773 0 $t Dissertation Abstracts International $g 63-07B.
790 $a 0168
790 1 0 $a Sayre, Richard T., $e advisor
791 $a Ph.D.
792 $a 2002
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3059324