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An examination of gut mucosal immuni...

Hebert, Pamela.

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An examination of gut mucosal immunity in the channel catfish.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	An examination of gut mucosal immunity in the channel catfish./
作者:	Hebert, Pamela.
面頁冊數:	120 p.
附註:	Major Professor: A. Jerald Ainsworth.
Contained By:	Dissertation Abstracts International60-09B.
標題:	Agriculture, Fisheries and Aquaculture. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9946325
ISBN:	9780599480605

An examination of gut mucosal immunity in the channel catfish.
Hebert, Pamela.

An examination of gut mucosal immunity in the channel catfish. - 120 p.

Major Professor: A. Jerald Ainsworth.

Thesis (Ph.D.)--Mississippi State University, 1999.

Taken together, these results suggest that there may be a paucity of humoral competent cells in the gut of channel catfish and that their gut immune defense is comprised of primarily innate responses.

ISBN: 9780599480605Subjects--Topical Terms:

1020913
Agriculture, Fisheries and Aquaculture.

An examination of gut mucosal immunity in the channel catfish.
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245 1 3 $a An examination of gut mucosal immunity in the channel catfish.
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500 $a Source: Dissertation Abstracts International, Volume: 60-09, Section: B, page: 4321.
502 $a Thesis (Ph.D.)--Mississippi State University, 1999.
520 $a Taken together, these results suggest that there may be a paucity of humoral competent cells in the gut of channel catfish and that their gut immune defense is comprised of primarily innate responses.
520 $a Channel catfish (CCF), Ictalurus punctatus, gut cells associated with innate or acquired immunity were characterized based on their reaction to monoclonal antibodies (mAb) using flow cytometry, histochemistry, immunohistochemistry, and enzyme staining techniques. Isolation of gut cells was accomplished by collagenase digestion to obtain cells from the lamina propria. Comparisons were performed by flow cytometry between cells obtained from the blood, gut, and head kidney. Results showed that the gut has a large proportion of neutrophils as compared to B lymphocytes.
520 $a Histochemical stains were used to determine the distribution and predicted function of cells present in the gut mucosa and submucosa. Stains included alpha-napthyl-butyrate esterase, alpha-napthyl-acetate esterase, acid phosphatase, beta-glucuronidase, Sudan Black B, methyl green pyronin, mucicarmine, and immunohistochemistry. These analyses were conducted by light microscopy using frozen or paraffin sections of gut tissues, and the results confirm flow cytometry data.
520 $a Experiments were undertaken in an effort to increase the serum antibody response to antigens administered to CCF via the gastrointestinal tract, compared to the responses induced by parenteral administration. Model soluble and particulate antigens were administered orally, anally, or intraperitoneally. Combinations of antigen alone, in addition to, or conjugated to cholera toxin or its B subunit were tested. Some antigen combinations were biopolymer coated. In addition, a viable viral vector was used to administer soluble antigen. Intraperitoneal treatment with particulate antigen alone, but not soluble antigen alone, induced antibody production above negative control values. Adjuvant-containing treatments were more effective than antigen alone, and fish receiving antigen-adjuvant conjugates with additional adjuvant had the highest serum antibody production. Serum antibody production in response to the viral vector was higher than controls and antigen alone in both oral and anal administration. It is therefore possible to favorably modulate serum antibody production in channel catfish following intraperitoneal, but not oral or anal, administration of both soluble and particulate antigens with the use of cholera toxin and a viral vector.
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650 4 $a Biology, Veterinary Science. $3 1021733
650 4 $a Health Sciences, Immunology. $3 1017716
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710 2 $a Mississippi State University. $3 1017550
773 0 $t Dissertation Abstracts International $g 60-09B.
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791 $a Ph.D.
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