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Low intensity pulsed ultrasound acce...
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Lu, Hongbin.
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Low intensity pulsed ultrasound accelerates bone-tendon junction healing.
Record Type:
Language materials, printed : Monograph/item
Title/Author:
Low intensity pulsed ultrasound accelerates bone-tendon junction healing./
Author:
Lu, Hongbin.
Description:
288 p.
Notes:
Advisers: Ling Qin; Kwok Sui Leung.
Contained By:
Dissertation Abstracts International68-03B.
Subject:
Health Sciences, Medicine and Surgery. -
Online resource:
http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3254576
Low intensity pulsed ultrasound accelerates bone-tendon junction healing.
Lu, Hongbin.
Low intensity pulsed ultrasound accelerates bone-tendon junction healing.
- 288 p.
Advisers: Ling Qin; Kwok Sui Leung.
Thesis (Ph.D.)--The Chinese University of Hong Kong (Hong Kong), 2006.
Keywords. partial patellectomy model; bone-tendon junction repair; low intensity pulsed ultrasound stimulations (LIPUS); gene expression; complementary DNA microarray; rabbit.Subjects--Topical Terms:
1017756
Health Sciences, Medicine and Surgery.
Low intensity pulsed ultrasound accelerates bone-tendon junction healing.
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Lu, Hongbin.
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Low intensity pulsed ultrasound accelerates bone-tendon junction healing.
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288 p.
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Advisers: Ling Qin; Kwok Sui Leung.
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Source: Dissertation Abstracts International, Volume: 68-03, Section: B, page: 1548.
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Thesis (Ph.D.)--The Chinese University of Hong Kong (Hong Kong), 2006.
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Keywords. partial patellectomy model; bone-tendon junction repair; low intensity pulsed ultrasound stimulations (LIPUS); gene expression; complementary DNA microarray; rabbit.
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This study explored the intact morphology, regular healing and the augmented healing under the effects of low intensity pulsed ultrasound stimulations (LIPUS) on the patella-patella tendon (PPT) junction in a rabbit partial patellectomy model. To probe its possible mechanism, the key genes involved in regulating osteogenesis mediated by LIPUS were identified using the state-of-the-art methods---complementary DNA microarray.
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Establishment of animal model for studying treatment efficacy of low-intensity pulsed ultrasound stimulations for accelerating bone-tendon repair. Standard partial patellectomy was conducted in the 18-week old rabbits that were then divided into the LIPUS treatment and control groups. The animals were followed for 2, 4, 8, and 16 weeks for various tissue analyses. LIPUS was applied to the experimental animals from postoperative day 3 to 16 weeks. We demonstrated that the healing process of PPT junction was initiated through endochondral ossification. The results showed that the size and length of newly formed bone, and its bone mineral content (BMC), but not its bone mineral density (BMD) were correlated with the failure load, ultimate strength and energy at failure. Using radiographic, biomechanical, histomorphologic and biomechanical methods, it was found that LIPUS had significant accelerating effect on PPT junction repair. We validated our study hypothesis in that LIPUS enhances bone-tendon junction healing by stimulating angiogenesis, chondrogenesis and osteogenesis.
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Establishment of in vitro model for mechanism study on effects of low-intensity pulsed ultrasound stimulations. An in vitro model of osteoblast-like cell line (SaOS-2 cells) was studied using cDNA microarray to explore the molecular mechanism mediated by LIPUS. This microarray analysis revealed a total of 165 genes that were regulated at 4 and 24 hours by LIPUS treatment in osteoblastic-like cells. These genes belonged to more than ten protein families based on their function and were involved in some signal transduction pathways. This study has validated the hypothesis that LIPUS can regulate a number of critical genes transient expressions in osteoblast cell line Saos-2.
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School code: 1307.
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Qin, Ling,
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http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3254576
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