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Bone protective effects of dried plu...

Bu, So Young.

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Bone protective effects of dried plum and its polyphenols under inflammatory and oxidative stress conditions.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Bone protective effects of dried plum and its polyphenols under inflammatory and oxidative stress conditions./
作者:	Bu, So Young.
面頁冊數:	216 p.
附註:	Adviser: Brenda J. Smith.
Contained By:	Dissertation Abstracts International68-04B.
標題:	Health Sciences, Nutrition. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3259592

Bone protective effects of dried plum and its polyphenols under inflammatory and oxidative stress conditions.
Bu, So Young.

Bone protective effects of dried plum and its polyphenols under inflammatory and oxidative stress conditions. - 216 p.

Adviser: Brenda J. Smith.

Thesis (Ph.D.)--Oklahoma State University, 2007.

Scope and method of study. The purpose of this study was to utilize the combination of in vivo and in vitro studies to investigate the effects of dried plum (DP) on bone and bone cell activity under conditions of elevated pro-inflammatory mediators. In Study 1, 9-month-old male osteopenic orchidectomized rats were randomly assigned to control (AIN-93M) or DP diet (25%, w/w) for 90 days. The positive control group consumed the control diet and received PTH (80mug/kg). In Study 2, macrophages (RAW 264.7) were pretreated with the polyphenols extracted from DP (0, 10, 20, or 30 mug/ml) cultured with or without RANKL and then stimulated with either LPS or hydrogen peroxide (H2O2). TRAP-positive osteoclasts and resorption pit formation were evaluated and inflammatory mediators such as NO and TNF-alpha by macrophages and during their differentiation into osteoclasts were assessed. In Study 3, MC3T3-E1 cells, murine pre-osteoblasts, were utilized to determine how DP polyphenols influence osteoblast activity, function and signaling under normal and inflammatory conditions. Cells were pretreated with polyphenols (0, 2.5, 5, 10 and 20 mug/ml) prior to stimulation with TNF-alpha (0 or 1.0 ng/ml). ALP activity and mineralized nodule formation were measured and alterations in gene expression of RANKL, OPG, Runx2, Osterix and IGF-I were analyzed.Subjects--Topical Terms:

1017801
Health Sciences, Nutrition.

Bone protective effects of dried plum and its polyphenols under inflammatory and oxidative stress conditions.
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100 1 $a Bu, So Young. $3 1265618
245 1 0 $a Bone protective effects of dried plum and its polyphenols under inflammatory and oxidative stress conditions.
300 $a 216 p.
500 $a Adviser: Brenda J. Smith.
500 $a Source: Dissertation Abstracts International, Volume: 68-04, Section: B, page: 2261.
502 $a Thesis (Ph.D.)--Oklahoma State University, 2007.
520 $a Scope and method of study. The purpose of this study was to utilize the combination of in vivo and in vitro studies to investigate the effects of dried plum (DP) on bone and bone cell activity under conditions of elevated pro-inflammatory mediators. In Study 1, 9-month-old male osteopenic orchidectomized rats were randomly assigned to control (AIN-93M) or DP diet (25%, w/w) for 90 days. The positive control group consumed the control diet and received PTH (80mug/kg). In Study 2, macrophages (RAW 264.7) were pretreated with the polyphenols extracted from DP (0, 10, 20, or 30 mug/ml) cultured with or without RANKL and then stimulated with either LPS or hydrogen peroxide (H2O2). TRAP-positive osteoclasts and resorption pit formation were evaluated and inflammatory mediators such as NO and TNF-alpha by macrophages and during their differentiation into osteoclasts were assessed. In Study 3, MC3T3-E1 cells, murine pre-osteoblasts, were utilized to determine how DP polyphenols influence osteoblast activity, function and signaling under normal and inflammatory conditions. Cells were pretreated with polyphenols (0, 2.5, 5, 10 and 20 mug/ml) prior to stimulation with TNF-alpha (0 or 1.0 ng/ml). ALP activity and mineralized nodule formation were measured and alterations in gene expression of RANKL, OPG, Runx2, Osterix and IGF-I were analyzed.
520 $a Findings and conclusions. Study 1 showed that dietary supplementation with DP restored the ORX-induced loss of bone mass and deterioration of bone structure. The restoration of bone microarchitecture by dried plum was comparable to PTH. Study 2 revealed that DP polyphenols inhibit osteoclastogenesis and osteoclast activity which may be mediated by the down-regulation of cytokines such as TNF-alpha and NO. Study 3 showed that DP polyphenols enhance osteoblast activity and mineralized nodule formation under normal and inflammatory conditions. Results in vitro studies suggest that dried plum which has ability to down-regulate inflammatory mediators, has potent effects on osteoclast and osteoblast activity and provide possible mechanisms by which dried plum preserves bone mass and microarchitecture as reported in current and previous in vivo studies.
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791 $a Ph.D.
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