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Defining the heterogeneity of protei...

Shandiz, Ali T.

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Defining the heterogeneity of protein transition states using new methods.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Defining the heterogeneity of protein transition states using new methods./
Author:	Shandiz, Ali T.
Description:	96 p.
Notes:	Adviser: Tobin R. Sosnick.
Contained By:	Dissertation Abstracts International68-02B.
Subject:	Biophysics, General. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3252246

Defining the heterogeneity of protein transition states using new methods.
Shandiz, Ali T.

Defining the heterogeneity of protein transition states using new methods. - 96 p.

Adviser: Tobin R. Sosnick.

Thesis (Ph.D.)--The University of Chicago, 2007.

The protein folding problem remains one of the fundamental mysteries of molecular biology. A continuing goal of experimental protein folding is to elucidate the structure of the transition state in the hopes of understanding the forces that drive the process.Subjects--Topical Terms:

1019105
Biophysics, General.

Defining the heterogeneity of protein transition states using new methods.
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005 20110512
008 110512s2007 ||||||||||||||||| ||eng d
035 $a (UMI)AAI3252246
035 $a AAI3252246
040 $a UMI $c UMI
100 1 $a Shandiz, Ali T. $3 1263218
245 1 0 $a Defining the heterogeneity of protein transition states using new methods.
300 $a 96 p.
500 $a Adviser: Tobin R. Sosnick.
500 $a Source: Dissertation Abstracts International, Volume: 68-02, Section: B, page: 0834.
502 $a Thesis (Ph.D.)--The University of Chicago, 2007.
520 $a The protein folding problem remains one of the fundamental mysteries of molecular biology. A continuing goal of experimental protein folding is to elucidate the structure of the transition state in the hopes of understanding the forces that drive the process.
520 $a First, we examine the utility of covalent crosslinking as a tool for inferring the extent of structure formation in the transition state ensemble. Although crosslinking appears to be prone to artifacts, possibly arising from native-state strain, conditions contributing to the occurrence of such artifacts have been identified. Crosslinking can be another useful tool for studying protein folding; provided it is employed under those circumstances it is most likely to be successful.
520 $a Second, we examine the diversity of the transition state ensemble of mammalian ubiquitin using a correlational approach employing multiple simultaneous perturbations. By introducing a crosslink at one site, while, its effect on the Psio-value at a second site is measured, we examined the correlations of sites that are fractionally populated in the transition state ensemble. We found that the TSE populations of the fractional sites examined were independent of each other and were unaffected by perturbations at distal sites. The results of these experiments indicated that a considerable amount of diversity is possible in the TSE of Ub, suggesting a possible entropic role for the fractional sites in stabilizing the TSE in folding.
590 $a School code: 0330.
650 4 $a Biophysics, General. $3 1019105
650 4 $a Chemistry, Biochemistry. $3 1017722
690 $a 0487
690 $a 0786
710 2 $a The University of Chicago. $3 1017389
773 0 $t Dissertation Abstracts International $g 68-02B.
790 $a 0330
790 1 0 $a Sosnick, Tobin R., $e advisor
791 $a Ph.D.
792 $a 2007
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3252246