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Microbial cell preservation in a sal...
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Janusz, Robert Emil.
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Microbial cell preservation in a saline-alkaline lake environment: A laboratory scale model.
紀錄類型:
書目-語言資料,印刷品 : Monograph/item
正題名/作者:
Microbial cell preservation in a saline-alkaline lake environment: A laboratory scale model./
作者:
Janusz, Robert Emil.
面頁冊數:
54 p.
附註:
Adviser: Stuart J. Birnbaum.
Contained By:
Masters Abstracts International46-05.
標題:
Biology, Microbiology. -
電子資源:
http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1450636
ISBN:
9780549510284
Microbial cell preservation in a saline-alkaline lake environment: A laboratory scale model.
Janusz, Robert Emil.
Microbial cell preservation in a saline-alkaline lake environment: A laboratory scale model.
- 54 p.
Adviser: Stuart J. Birnbaum.
Thesis (M.S.)--The University of Texas at San Antonio, 2008.
A laboratory scale model based on Natrialba magadii , a haloalkaliphilic archaea isolated from the saline-alkaline brine of Lake Magadi in the East African Rift Zone, was employed to investigate whether erionite, the most common zeolite mineral phase found in the trona producing areas in the lake, influences the preservation of microbial cells during evaporation. Erionite has a high cation exchange capacity and a strong affinity for K+ (Pabalan and Bertetti, 2001) and its structure contains exchangeable H2O. Haloalkaliphilic archea accumulate K + to maintain osmotic balance. Therefore, the archean cells cultured in the presence of erionite may access K+ and H2O in the erionite to maintain osmotic balance as the brine precipitates evaporite mineral phases when evaporating to dryness. Liquid media cultures of Natrialba magadii, in the presence and absence of erionite, were allowed to evaporate to dryness under aseptic conditions in a laboratory incubator. Mineral precipitates were analyzed using scanning electron microscopy, energy dispersive x-ray analysis and x-ray powder diffraction.
ISBN: 9780549510284Subjects--Topical Terms:
1017734
Biology, Microbiology.
Microbial cell preservation in a saline-alkaline lake environment: A laboratory scale model.
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A laboratory scale model based on Natrialba magadii , a haloalkaliphilic archaea isolated from the saline-alkaline brine of Lake Magadi in the East African Rift Zone, was employed to investigate whether erionite, the most common zeolite mineral phase found in the trona producing areas in the lake, influences the preservation of microbial cells during evaporation. Erionite has a high cation exchange capacity and a strong affinity for K+ (Pabalan and Bertetti, 2001) and its structure contains exchangeable H2O. Haloalkaliphilic archea accumulate K + to maintain osmotic balance. Therefore, the archean cells cultured in the presence of erionite may access K+ and H2O in the erionite to maintain osmotic balance as the brine precipitates evaporite mineral phases when evaporating to dryness. Liquid media cultures of Natrialba magadii, in the presence and absence of erionite, were allowed to evaporate to dryness under aseptic conditions in a laboratory incubator. Mineral precipitates were analyzed using scanning electron microscopy, energy dispersive x-ray analysis and x-ray powder diffraction.
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Upon analysis, microcosms containing both N. magadii culture and erionite revealed preserved cells. No preserved cells were seen in microcosms containing only the culture in liquid media. This observed relationship between erionite and preserved microbial "fossils" sheds light on the geomicrobiology of Natrialba magadii and zeolites and specifically the association with potassium cycling. This relationship may have significance in the study of ion exchange in zeolitic soil and water bearing zeolite rich rock substrate under saline-alkaline conditions.
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