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Microaerobic fermentation of enginee...

Tsuruta, Hiroko.

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Microaerobic fermentation of engineered Escherichia coli for the production of biofuels.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Microaerobic fermentation of engineered Escherichia coli for the production of biofuels./
作者:	Tsuruta, Hiroko.
面頁冊數:	108 p.
附註:	Adviser: Gregory L. Young.
Contained By:	Masters Abstracts International46-04.
標題:	Biology, Microbiology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1452046
ISBN:	9780549464228

Microaerobic fermentation of engineered Escherichia coli for the production of biofuels.
Tsuruta, Hiroko.

Microaerobic fermentation of engineered Escherichia coli for the production of biofuels. - 108 p.

Adviser: Gregory L. Young.

Thesis (M.S.)--San Jose State University, 2007.

Aerobic, microaerobic, and anaerobic batch cultivation of E. coli for heterologous production of mevalonate were performed in bench-scale bioreactors. To screen different degrees of microaerobicity, airflow was kept constant and the amount of oxygen fed into the bioreactor was varied by changing the percentage of air in a gas mixture. Glucose consumption, cell growth, mevalonate and organic acids were measured and used to determine the yield of mevalonate on glucose. The study showed different distributions of carbon into products. Under aerobic cultivation, the majority of glucose was converted into biomass and CO2. Anaerobic cultivation made a small amount of mevalonate but primarily produced organic acids and ethanol. At low microaerobic range, glucose was converted into organic acids which accumulate due to the lack of an electron acceptor. At high microaerobic range, organic acids made from glucose were further metabolized to form additional mevalonate and yielded the highest conversion of mevalonate from glucose.

ISBN: 9780549464228Subjects--Topical Terms:

1017734
Biology, Microbiology.

Microaerobic fermentation of engineered Escherichia coli for the production of biofuels.
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