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Translation misreading in lacZ gene ...

Nag, Nabanita.

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Translation misreading in lacZ gene in Escherichia coli.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Translation misreading in lacZ gene in Escherichia coli./
Author:	Nag, Nabanita.
Description:	99 p.
Notes:	Adviser: Philip J. Farabaugh.
Contained By:	Masters Abstracts International46-03.
Subject:	Biology, General. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1447111
ISBN:	9780549271734

Translation misreading in lacZ gene in Escherichia coli.
Nag, Nabanita.

Translation misreading in lacZ gene in Escherichia coli. - 99 p.

Adviser: Philip J. Farabaugh.

Thesis (M.S.)--University of Maryland, Baltimore County, 2007.

One type of error that can occur during translation of a messenger RNA is a misreading error. Misreading happens in the ribosomal A site when there is a mismatch between the codon of the mRNA and the anticodon of the tRNA. Estimates of the missense error frequency have varied between 10-3 to 10-4 per codon. Several studies have been done using different methods and reported varying error rates, which makes it difficult to compare them. In this study I have used a system to develop estimates for the range of error rates more accurately in E.coli. I have introduced mutations affecting two essential residues, glutamic acid 537 (E537) and histidine 391 (H391), in the active site of the enzyme beta-galactosidase. These two residues form part of the active site and interact with the bound substrate. Most mutations at the E537 codon reduce enzyme activity to 3-5 x 10-5 the level of wild type. At H391, most reduce activity to 4-9 x 10-5 of wild type. Some mutants, however, have much higher activity, up to 100-fold higher for some H391 mutations and 10-fold for some E537 mutations. I hypothesized that the enzyme activity of these mutants is due to the misreading by tRNAGLU and tRNAHisGUA . My study reports on the missense error rates of a large number of near-cognate codons in these two residues. I also used aminoglycoside antibiotics and ribosomal protein mutations (S4, S5 and S12) to study their effects on these near and non-cognate codons and found that they affect misreading on only some near-cognate codons. These results suggest that the effect of these ribosomal protein mutations and aminoglycoside antibiotics in translation misreading are less general than has been previously reported.

ISBN: 9780549271734Subjects--Topical Terms:

1018625
Biology, General.

Translation misreading in lacZ gene in Escherichia coli.
LDR:02575nam 2200277 a 45 001 939171
005 20110512
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020 $a 9780549271734
035 $a (UMI)AAI1447111
035 $a AAI1447111
040 $a UMI $c UMI
100 1 $a Nag, Nabanita. $3 1263162
245 1 0 $a Translation misreading in lacZ gene in Escherichia coli.
300 $a 99 p.
500 $a Adviser: Philip J. Farabaugh.
500 $a Source: Masters Abstracts International, Volume: 46-03, page: 1367.
502 $a Thesis (M.S.)--University of Maryland, Baltimore County, 2007.
520 $a One type of error that can occur during translation of a messenger RNA is a misreading error. Misreading happens in the ribosomal A site when there is a mismatch between the codon of the mRNA and the anticodon of the tRNA. Estimates of the missense error frequency have varied between 10-3 to 10-4 per codon. Several studies have been done using different methods and reported varying error rates, which makes it difficult to compare them. In this study I have used a system to develop estimates for the range of error rates more accurately in E.coli. I have introduced mutations affecting two essential residues, glutamic acid 537 (E537) and histidine 391 (H391), in the active site of the enzyme beta-galactosidase. These two residues form part of the active site and interact with the bound substrate. Most mutations at the E537 codon reduce enzyme activity to 3-5 x 10-5 the level of wild type. At H391, most reduce activity to 4-9 x 10-5 of wild type. Some mutants, however, have much higher activity, up to 100-fold higher for some H391 mutations and 10-fold for some E537 mutations. I hypothesized that the enzyme activity of these mutants is due to the misreading by tRNAGLU and tRNAHisGUA . My study reports on the missense error rates of a large number of near-cognate codons in these two residues. I also used aminoglycoside antibiotics and ribosomal protein mutations (S4, S5 and S12) to study their effects on these near and non-cognate codons and found that they affect misreading on only some near-cognate codons. These results suggest that the effect of these ribosomal protein mutations and aminoglycoside antibiotics in translation misreading are less general than has been previously reported.
590 $a School code: 0434.
650 4 $a Biology, General. $3 1018625
650 4 $a Biology, Molecular. $3 1017719
690 $a 0306
690 $a 0307
710 2 $a University of Maryland, Baltimore County. $b Biological Sciences. $3 1263163
773 0 $t Masters Abstracts International $g 46-03.
790 $a 0434
790 1 0 $a Farabaugh, Philip J., $e advisor
791 $a M.S.
792 $a 2007
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1447111