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Translation misreading in lacZ gene ...

Nag, Nabanita.

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Translation misreading in lacZ gene in Escherichia coli.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Translation misreading in lacZ gene in Escherichia coli./
作者:	Nag, Nabanita.
面頁冊數:	99 p.
附註:	Adviser: Philip J. Farabaugh.
Contained By:	Masters Abstracts International46-03.
標題:	Biology, General. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=1447111
ISBN:	9780549271734

Translation misreading in lacZ gene in Escherichia coli.
Nag, Nabanita.

Translation misreading in lacZ gene in Escherichia coli. - 99 p.

Adviser: Philip J. Farabaugh.

Thesis (M.S.)--University of Maryland, Baltimore County, 2007.

One type of error that can occur during translation of a messenger RNA is a misreading error. Misreading happens in the ribosomal A site when there is a mismatch between the codon of the mRNA and the anticodon of the tRNA. Estimates of the missense error frequency have varied between 10-3 to 10-4 per codon. Several studies have been done using different methods and reported varying error rates, which makes it difficult to compare them. In this study I have used a system to develop estimates for the range of error rates more accurately in E.coli. I have introduced mutations affecting two essential residues, glutamic acid 537 (E537) and histidine 391 (H391), in the active site of the enzyme beta-galactosidase. These two residues form part of the active site and interact with the bound substrate. Most mutations at the E537 codon reduce enzyme activity to 3-5 x 10-5 the level of wild type. At H391, most reduce activity to 4-9 x 10-5 of wild type. Some mutants, however, have much higher activity, up to 100-fold higher for some H391 mutations and 10-fold for some E537 mutations. I hypothesized that the enzyme activity of these mutants is due to the misreading by tRNAGLU and tRNAHisGUA . My study reports on the missense error rates of a large number of near-cognate codons in these two residues. I also used aminoglycoside antibiotics and ribosomal protein mutations (S4, S5 and S12) to study their effects on these near and non-cognate codons and found that they affect misreading on only some near-cognate codons. These results suggest that the effect of these ribosomal protein mutations and aminoglycoside antibiotics in translation misreading are less general than has been previously reported.

ISBN: 9780549271734Subjects--Topical Terms:

1018625
Biology, General.

Translation misreading in lacZ gene in Escherichia coli.
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520 $a One type of error that can occur during translation of a messenger RNA is a misreading error. Misreading happens in the ribosomal A site when there is a mismatch between the codon of the mRNA and the anticodon of the tRNA. Estimates of the missense error frequency have varied between 10-3 to 10-4 per codon. Several studies have been done using different methods and reported varying error rates, which makes it difficult to compare them. In this study I have used a system to develop estimates for the range of error rates more accurately in E.coli. I have introduced mutations affecting two essential residues, glutamic acid 537 (E537) and histidine 391 (H391), in the active site of the enzyme beta-galactosidase. These two residues form part of the active site and interact with the bound substrate. Most mutations at the E537 codon reduce enzyme activity to 3-5 x 10-5 the level of wild type. At H391, most reduce activity to 4-9 x 10-5 of wild type. Some mutants, however, have much higher activity, up to 100-fold higher for some H391 mutations and 10-fold for some E537 mutations. I hypothesized that the enzyme activity of these mutants is due to the misreading by tRNAGLU and tRNAHisGUA . My study reports on the missense error rates of a large number of near-cognate codons in these two residues. I also used aminoglycoside antibiotics and ribosomal protein mutations (S4, S5 and S12) to study their effects on these near and non-cognate codons and found that they affect misreading on only some near-cognate codons. These results suggest that the effect of these ribosomal protein mutations and aminoglycoside antibiotics in translation misreading are less general than has been previously reported.
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