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From capillary to microchip electrop...

Gawron, Andrew Joseph.

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From capillary to microchip electrophoresis: Electrochemical detection of peptides.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	From capillary to microchip electrophoresis: Electrochemical detection of peptides./
作者:	Gawron, Andrew Joseph.
面頁冊數:	235 p.
附註:	Chair: Susan M. Lunte.
Contained By:	Dissertation Abstracts International62-06B.
標題:	Chemistry, Pharmaceutical. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3018496
ISBN:	0493290559

From capillary to microchip electrophoresis: Electrochemical detection of peptides.
Gawron, Andrew Joseph.

From capillary to microchip electrophoresis: Electrochemical detection of peptides. - 235 p.

Chair: Susan M. Lunte.

Thesis (Ph.D.)--University of Kansas, 2001.

The ability to study metabolism, transport, and release of peptides at biologically relevant levels is an enormous analytical challenge. This dissertation addresses that challenge in two parts. The first part describes the development of selective analytical methodology for peptides using copper complexation and conventional capillary electrophoresis (CE) with electrochemical detection. Preliminary work using CE with UV detection revealed that copper-peptide complexes could be formed on-capillary, by adding copper to the run buffer, or precapillary by adding copper to the sample. Further work was done to characterize the on-capillary technique using CE with single electrode detection to monitor the oxidation of the complex. Numerous peptides were evaluated to determine the applicability of the method, including angiotensin I, II, and III, angiotensin 1–7, oxytocin, des-Tyr leu-enkephalin, and leu-enkephalin. The utility of on-capillary copper complexation for the analysis of a biological sample was confirmed by monitoring the degradation of leu-enkephalin and the formation of its metabolite des-Tyr leu-enkephalin in plasma.

ISBN: 0493290559Subjects--Topical Terms:

550957
Chemistry, Pharmaceutical.

From capillary to microchip electrophoresis: Electrochemical detection of peptides.
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245 1 0 $a From capillary to microchip electrophoresis: Electrochemical detection of peptides.
300 $a 235 p.
500 $a Chair: Susan M. Lunte.
500 $a Source: Dissertation Abstracts International, Volume: 62-06, Section: B, page: 2731.
502 $a Thesis (Ph.D.)--University of Kansas, 2001.
520 $a The ability to study metabolism, transport, and release of peptides at biologically relevant levels is an enormous analytical challenge. This dissertation addresses that challenge in two parts. The first part describes the development of selective analytical methodology for peptides using copper complexation and conventional capillary electrophoresis (CE) with electrochemical detection. Preliminary work using CE with UV detection revealed that copper-peptide complexes could be formed on-capillary, by adding copper to the run buffer, or precapillary by adding copper to the sample. Further work was done to characterize the on-capillary technique using CE with single electrode detection to monitor the oxidation of the complex. Numerous peptides were evaluated to determine the applicability of the method, including angiotensin I, II, and III, angiotensin 1–7, oxytocin, des-Tyr leu-enkephalin, and leu-enkephalin. The utility of on-capillary copper complexation for the analysis of a biological sample was confirmed by monitoring the degradation of leu-enkephalin and the formation of its metabolite des-Tyr leu-enkephalin in plasma.
520 $a Since the copper complexes exhibit a chemically reversible redox reaction, dual-electrode detection provides a means of selectively detecting these compounds. The second focus of this thesis was the development of microchip CE systems with integrated dual-electrode electrochemical detectors for the analysis of copper-peptide complexes. A silicon based master was used to fabricate the separation channels from poly(dimethylsiloxane) (PDMS). The electrodes were fabricated using photolithography (Au/Cr) or by simply incorporating the electrode material (carbon fiber) within a separate layer of PDMS. The separation and electrode layers were then sealed together to construct a truly miniature analytical system with an integrated electrochemical detector. The analytical performance and limitations of these microchip CE systems are presented. This dissertation includes the first report of dual-electrode electrochemical detection with microchip CE. Dual-electrode detection of copper-peptide complexes was accomplished with both carbon fiber and paste electrochemical detectors.
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