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Recognition and oxidative strand sci...

Humphreys, Kristi Jean.

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Recognition and oxidative strand scission of nucleic acids by multinuclear copper(II) coordination complexes.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Recognition and oxidative strand scission of nucleic acids by multinuclear copper(II) coordination complexes./
Author:	Humphreys, Kristi Jean.
Description:	184 p.
Notes:	Adviser: Kenneth D. Carlin.
Contained By:	Dissertation Abstracts International62-10B.
Subject:	Chemistry, Biochemistry. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3028284
ISBN:	0493403353

Recognition and oxidative strand scission of nucleic acids by multinuclear copper(II) coordination complexes.
Humphreys, Kristi Jean.

Recognition and oxidative strand scission of nucleic acids by multinuclear copper(II) coordination complexes. - 184 p.

Adviser: Kenneth D. Carlin.

Thesis (Ph.D.)--The Johns Hopkins University, 2002.

The compound [Cu2<super>II</super>(<bold>D</bold><super>1 </super>)(H2O)2](ClO4)4·2H 2O mediates efficient oxidative cleavage of DNA under conditions conducive for reduction of the metal centers and subsequent activation of dioxygen. Relative to a mononuclear analogue, the binuclear complex more rapidly effects degradation of both plasmids and oligonucleotides. Cleavage requires both the complex and a reductant and likely proceeds through a nondiffusible O 2-derived intermediate, the formation of which may be dependent on the presence of multiple metal ions in the same complex.

ISBN: 0493403353Subjects--Topical Terms:

1017722
Chemistry, Biochemistry.

Recognition and oxidative strand scission of nucleic acids by multinuclear copper(II) coordination complexes.
LDR:03770nam 2200301 a 45 001 935311
005 20110509
008 110509s2002 eng d
020 $a 0493403353
035 $a (UnM)AAI3028284
035 $a AAI3028284
040 $a UnM $c UnM
100 1 $a Humphreys, Kristi Jean. $3 1259000
245 1 0 $a Recognition and oxidative strand scission of nucleic acids by multinuclear copper(II) coordination complexes.
300 $a 184 p.
500 $a Adviser: Kenneth D. Carlin.
500 $a Source: Dissertation Abstracts International, Volume: 62-10, Section: B, page: 4536.
502 $a Thesis (Ph.D.)--The Johns Hopkins University, 2002.
520 $a The compound [Cu2<super>II</super>(<bold>D</bold><super>1 </super>)(H2O)2](ClO4)4·2H 2O mediates efficient oxidative cleavage of DNA under conditions conducive for reduction of the metal centers and subsequent activation of dioxygen. Relative to a mononuclear analogue, the binuclear complex more rapidly effects degradation of both plasmids and oligonucleotides. Cleavage requires both the complex and a reductant and likely proceeds through a nondiffusible O 2-derived intermediate, the formation of which may be dependent on the presence of multiple metal ions in the same complex.
520 $a A trinuclear copper complex, [Cu3<super>II</super>(<bold> L</bold>)(H2O)3(NO3)2](NO 3)4·5H2O, exhibits a remarkable ability to promote specific strand scission at helix/coil junctions. Strand scission occurs on the 3<super>′</super> overhang at the junction of a hairpin or frayed duplex structure and is not dependent on the identity of the base at which cleavage occurs. Target recognition minimally requires a purine at the first unpaired position and a guanine at the second unpaired position on the 5<super>′</super> extension. Selective strand scission requires both a reductant and dioxygen and does not appear to involve a diffusible radical. Comparisons between the trinuclear copper complex and mononuclear compounds indicate that the recognition and reactivity described in this report are also dependent on the multinuclearity of the complex.
520 $a The compound [Cu2<super>II</super>(<bold>D</bold><super>1 </super>)(H2O)2](ClO4)4 also selectively promotes cleavage of DNA on nucleotide strands that extend from the 3<super> ′</super> side of frayed duplex structures at a site two residues displaced from the junction. The minimal requirements for reaction include a guanine in the first unpaired position of the 3<super>′</super> overhang adjacent to the cleavage site and an adenine in the parallel position on the 5<super> ′</super> overhang. Recognition and strand scission are independent of the nucleobase at the cleavage site. The necessary presence of both a reductant and dioxygen indicates that the intermediate responsible for cleavage is produced by the activation of dioxygen by a copper(I) form of the binuclear complex. The unique coordination and reactivity properties of [Cu2<super> II</super>(<bold>D</bold><super>1</super>)(H2O)2](ClO 4)4 are critical for its efficiency and site selectivity since mononuclear compounds and a binuclear analogue exhibit only nonselective cleavage of DNA.
590 $a School code: 0098.
650 4 $a Chemistry, Biochemistry. $3 1017722
650 4 $a Chemistry, Inorganic. $3 517253
690 $a 0487
690 $a 0488
710 2 0 $a The Johns Hopkins University. $3 1017431
773 0 $t Dissertation Abstracts International $g 62-10B.
790 $a 0098
790 1 0 $a Carlin, Kenneth D., $e advisor
791 $a Ph.D.
792 $a 2002
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3028284