東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

Development of the zebrafish dorsal ...

Ungos, Josette Marie.

FindBook

Google Book

Amazon

博客來

Development of the zebrafish dorsal root ganglia: The role of Shh signaling, Neurogenin1 and sensory deprived in specification of DRG neurons.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Development of the zebrafish dorsal root ganglia: The role of Shh signaling, Neurogenin1 and sensory deprived in specification of DRG neurons./
作者:	Ungos, Josette Marie.
面頁冊數:	129 p.
附註:	Chair: David W. Raible.
Contained By:	Dissertation Abstracts International63-08B.
標題:	Biology, Genetics. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3063028
ISBN:	0493815716

Development of the zebrafish dorsal root ganglia: The role of Shh signaling, Neurogenin1 and sensory deprived in specification of DRG neurons.
Ungos, Josette Marie.

Development of the zebrafish dorsal root ganglia: The role of Shh signaling, Neurogenin1 and sensory deprived in specification of DRG neurons. - 129 p.

Chair: David W. Raible.

Thesis (Ph.D.)--University of Washington, 2002.

The dorsal root ganglia (DRG) are the sensory ganglia that comprise the main somatosensory system in vertebrate organisms. Neurons and glia of the DRG derive from trunk and tail neural crest cells that coalesce at the lateral edge of the spinal cord. The mechanisms by which neural crest cells are specified to adopt a DRG sensory fate are poorly understood. The work presented in this thesis defines several factors important for specification of zebrafish (<italic> Danio rerio</italic>) DRG neurons. I demonstrate that the basic helix-loop-helix transcription factor, Neurogenin1 (Ngn1), is generally required for the specification of sensory neurons, including DRG neurons. <italic>ngn1</italic> is expressed in DRG precursors at the site of the nascent ganglion. Anti-sense morpholino-mediated knockdown of zebrafish Ngn1 function results in the complete absence of DRG neurons as well as loss of cranial ganglia and Rohon-Beard primary sensory neurons. In comparison, I show that <italic>sensory deprived</italic> (<italic> sdp</italic>) is required for the development specifically of DRG neurons. The zebrafish <italic>sdp</italic> mutation results in the complete loss of DRG neurons yet other sensory neurons are unaffected and other neural crest derivatives develop normally. Moreover, I show that <italic>sdp</italic> is required for the expression of <italic>ngn1</italic> in DRG precursors. Through analysis of several zebrafish mutants and through use of a pharmacological inhibitor, I show that Sonic hedgehog (Shh) signaling is also necessary for the development of DRG neurons. This requirement to receive a Shh signal is cell autonomous with respect to DRG precursors and occurs prior to the onset of <italic>ngn1</italic> expression in the nascent ganglion. DRG expression of <italic>ngn1</italic> is abolished in the Shh signaling mutants. Finally, I provide evidence for an additional role for Shh signaling during DRG development. Although loss of Shh signaling results in absence of DRG neurons, in some segments I also observe ganglia containing up to tenfold more neurons than normal ganglia. These abnormally large ganglia form in the absence of either Ngn1 or <italic>sdp</italic> function suggesting that these neurons may derive from a separate DRG precursor population that is normally repressed by Shh signaling.

ISBN: 0493815716Subjects--Topical Terms:

1017730
Biology, Genetics.

Development of the zebrafish dorsal root ganglia: The role of Shh signaling, Neurogenin1 and sensory deprived in specification of DRG neurons.
LDR:03310nam 2200289 a 45 001 934685
005 20110509
008 110509s2002 eng d
020 $a 0493815716
035 $a (UnM)AAI3063028
035 $a AAI3063028
040 $a UnM $c UnM
100 1 $a Ungos, Josette Marie. $3 1258384
245 1 0 $a Development of the zebrafish dorsal root ganglia: The role of Shh signaling, Neurogenin1 and sensory deprived in specification of DRG neurons.
300 $a 129 p.
500 $a Chair: David W. Raible.
500 $a Source: Dissertation Abstracts International, Volume: 63-08, Section: B, page: 3606.
502 $a Thesis (Ph.D.)--University of Washington, 2002.
520 $a The dorsal root ganglia (DRG) are the sensory ganglia that comprise the main somatosensory system in vertebrate organisms. Neurons and glia of the DRG derive from trunk and tail neural crest cells that coalesce at the lateral edge of the spinal cord. The mechanisms by which neural crest cells are specified to adopt a DRG sensory fate are poorly understood. The work presented in this thesis defines several factors important for specification of zebrafish (<italic> Danio rerio</italic>) DRG neurons. I demonstrate that the basic helix-loop-helix transcription factor, Neurogenin1 (Ngn1), is generally required for the specification of sensory neurons, including DRG neurons. <italic>ngn1</italic> is expressed in DRG precursors at the site of the nascent ganglion. Anti-sense morpholino-mediated knockdown of zebrafish Ngn1 function results in the complete absence of DRG neurons as well as loss of cranial ganglia and Rohon-Beard primary sensory neurons. In comparison, I show that <italic>sensory deprived</italic> (<italic> sdp</italic>) is required for the development specifically of DRG neurons. The zebrafish <italic>sdp</italic> mutation results in the complete loss of DRG neurons yet other sensory neurons are unaffected and other neural crest derivatives develop normally. Moreover, I show that <italic>sdp</italic> is required for the expression of <italic>ngn1</italic> in DRG precursors. Through analysis of several zebrafish mutants and through use of a pharmacological inhibitor, I show that Sonic hedgehog (Shh) signaling is also necessary for the development of DRG neurons. This requirement to receive a Shh signal is cell autonomous with respect to DRG precursors and occurs prior to the onset of <italic>ngn1</italic> expression in the nascent ganglion. DRG expression of <italic>ngn1</italic> is abolished in the Shh signaling mutants. Finally, I provide evidence for an additional role for Shh signaling during DRG development. Although loss of Shh signaling results in absence of DRG neurons, in some segments I also observe ganglia containing up to tenfold more neurons than normal ganglia. These abnormally large ganglia form in the absence of either Ngn1 or <italic>sdp</italic> function suggesting that these neurons may derive from a separate DRG precursor population that is normally repressed by Shh signaling.
590 $a School code: 0250.
650 4 $a Biology, Genetics. $3 1017730
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Biology, Neuroscience. $3 1017680
690 $a 0307
690 $a 0317
690 $a 0369
710 2 0 $a University of Washington. $3 545923
773 0 $t Dissertation Abstracts International $g 63-08B.
790 $a 0250
790 1 0 $a Raible, David W., $e advisor
791 $a Ph.D.
792 $a 2002
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3063028