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Forward and reverse chemical genetic...

Stockwell, Brent Roark.

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Forward and reverse chemical genetic studies of transforming growth factor beta signaling.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Forward and reverse chemical genetic studies of transforming growth factor beta signaling./
作者:	Stockwell, Brent Roark.
面頁冊數:	137 p.
附註:	Adviser: Stuart L. Schreiber.
Contained By:	Dissertation Abstracts International60-11B.
標題:	Chemistry, Biochemistry. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9949810
ISBN:	0599522046

Forward and reverse chemical genetic studies of transforming growth factor beta signaling.
Stockwell, Brent Roark.

Forward and reverse chemical genetic studies of transforming growth factor beta signaling. - 137 p.

Adviser: Stuart L. Schreiber.

Thesis (Ph.D.)--Harvard University, 1999.

A forward genetic approach involves phenotype-based screens for genes affecting a process of interest, whereas a reverse genetic approach involves alteration of a particular gene and description of the resulting phenotype. These approaches, when extended to functional perturbations caused by small molecules instead of mutations, are termed forward and reverse chemical genetics. We applied these methods of analysis to transforming growth factor beta (TGF-β) signaling.

ISBN: 0599522046Subjects--Topical Terms:

1017722
Chemistry, Biochemistry.

Forward and reverse chemical genetic studies of transforming growth factor beta signaling.
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245 1 0 $a Forward and reverse chemical genetic studies of transforming growth factor beta signaling.
300 $a 137 p.
500 $a Adviser: Stuart L. Schreiber.
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502 $a Thesis (Ph.D.)--Harvard University, 1999.
520 $a A forward genetic approach involves phenotype-based screens for genes affecting a process of interest, whereas a reverse genetic approach involves alteration of a particular gene and description of the resulting phenotype. These approaches, when extended to functional perturbations caused by small molecules instead of mutations, are termed forward and reverse chemical genetics. We applied these methods of analysis to transforming growth factor beta (TGF-β) signaling.
520 $a We used a reverse chemical genetic approach to study the TGF-β receptors. Thus, small molecules were used to activate the TGF-β receptors conditionally. The role of each receptor in inducing the phenotypic changes caused by TGF-β was assessed. Such phenotypic changes included Smad2 nuclear translocation, reporter gene activation, and growth inhibition of mink lung cells.
520 $a We also used a forward chemical genetic approach to study TGF-β signaling. We developed high throughput assays for small molecule modifiers of TGF-β signaling, including a generally useful antibody-based assay referred to as a cytoblot. We identified small molecule activators of a TGF-β-responsive reporter gene, and demonstrated the effectiveness of whole-genome transcriptional profiling to study the mechanisms by which these small molecules act.
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