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Oxidative stress and lipid catabolis...

Zabrouskov, Vladimir.

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Oxidative stress and lipid catabolism during aging of potato seed-tubers.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Oxidative stress and lipid catabolism during aging of potato seed-tubers./
作者:	Zabrouskov, Vladimir.
面頁冊數:	153 p.
附註:	Chair: Norman R. Knowles.
Contained By:	Dissertation Abstracts International63-07B.
標題:	Biology, Plant Physiology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3058825
ISBN:	0493745289

Oxidative stress and lipid catabolism during aging of potato seed-tubers.
Zabrouskov, Vladimir.

Oxidative stress and lipid catabolism during aging of potato seed-tubers. - 153 p.

Chair: Norman R. Knowles.

Thesis (Ph.D.)--Washington State University, 2002.

The role of lipid metabolism in the development of oxidative stress and loss of growth potential during aging of potato tubers was examined. Transgenic tubers containing high levels of α-linolenic acid underwent accelerated aging, resulting in growth responses characteristic of older tubers. Relative to wild-type tubers, aging of high α-linolenate tubers resulted in increased tuber respiration, increased membrane permeability, and higher levels of lipid peroxidation. Thus, by affecting the susceptibility of lipids to peroxidation, the degree of fatty acid unsaturation influenced the development of oxidative stress and the overall rate at which growth potential was lost from tubers during aging. A method for quantifying lipid molecular species in biological matrices using MALDI-TOF mass spectrometry was developed. Using this method changes in polar lipid molecular species and sterols were characterized in microsomes as a prerequisite to determining how the lipid chemistry of membranes affects susceptibility to peroxidation during aging. Selective changes in lipid molecular species revealed differential susceptibilities of phospho- and galactolipids to age-induced catabolism. In general, aging effected significant increases in 18:3-bearing phospholipids that may have increased the susceptibility of membranes to peroxidative damage. The microsomal and mitochondrial polar lipids of high α-linolenate tubers were dominated by 18:3/18:3 and 16:0/18:3 species. Age-induced decline in 18:3-bearing phosphatidylcholines was partially offset by increases in 18:3-bearing phosphatidylethanolamines in mitochondrial membranes of high α-linolenate tubers. Interestingly, these age and transformation-induced changes had no effect on the integrity and function of mitochondria <italic>in vitro</italic>. Hence, differential increases in the respiration rates of wild-type and high α-linolenate tubers during aging were at least partly a consequence of unsaturation-dependent differences in the energy demands for free radical scavenging through the ascorbate-glutathione antioxidant pathway.

ISBN: 0493745289Subjects--Topical Terms:

1017865
Biology, Plant Physiology.

Oxidative stress and lipid catabolism during aging of potato seed-tubers.
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520 $a The role of lipid metabolism in the development of oxidative stress and loss of growth potential during aging of potato tubers was examined. Transgenic tubers containing high levels of α-linolenic acid underwent accelerated aging, resulting in growth responses characteristic of older tubers. Relative to wild-type tubers, aging of high α-linolenate tubers resulted in increased tuber respiration, increased membrane permeability, and higher levels of lipid peroxidation. Thus, by affecting the susceptibility of lipids to peroxidation, the degree of fatty acid unsaturation influenced the development of oxidative stress and the overall rate at which growth potential was lost from tubers during aging. A method for quantifying lipid molecular species in biological matrices using MALDI-TOF mass spectrometry was developed. Using this method changes in polar lipid molecular species and sterols were characterized in microsomes as a prerequisite to determining how the lipid chemistry of membranes affects susceptibility to peroxidation during aging. Selective changes in lipid molecular species revealed differential susceptibilities of phospho- and galactolipids to age-induced catabolism. In general, aging effected significant increases in 18:3-bearing phospholipids that may have increased the susceptibility of membranes to peroxidative damage. The microsomal and mitochondrial polar lipids of high α-linolenate tubers were dominated by 18:3/18:3 and 16:0/18:3 species. Age-induced decline in 18:3-bearing phosphatidylcholines was partially offset by increases in 18:3-bearing phosphatidylethanolamines in mitochondrial membranes of high α-linolenate tubers. Interestingly, these age and transformation-induced changes had no effect on the integrity and function of mitochondria <italic>in vitro</italic>. Hence, differential increases in the respiration rates of wild-type and high α-linolenate tubers during aging were at least partly a consequence of unsaturation-dependent differences in the energy demands for free radical scavenging through the ascorbate-glutathione antioxidant pathway.
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