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Community analysis of an anaerobic t...

Dennis, Philip Christopher.

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Community analysis of an anaerobic tetrachloroethene (PCE) degrading bacterial consortium.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Community analysis of an anaerobic tetrachloroethene (PCE) degrading bacterial consortium./
作者:	Dennis, Philip Christopher.
面頁冊數:	144 p.
附註:	Advisers: Brent Sleep; Steven Liss.
Contained By:	Masters Abstracts International39-02.
標題:	Biology, Microbiology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=MQ53371
ISBN:	0612533719

Community analysis of an anaerobic tetrachloroethene (PCE) degrading bacterial consortium.
Dennis, Philip Christopher.

Community analysis of an anaerobic tetrachloroethene (PCE) degrading bacterial consortium. - 144 p.

Advisers: Brent Sleep; Steven Liss.

Thesis (M.A.Sc.)--University of Toronto (Canada), 2000.

The community structure of an anaerobic microbial consortium, capable of dechlorination of saturation levels of tetrachloroethene in an continuous-flow column was examined via biomass analysis, comparative 16S rRNA analysis, ARDRA, and denaturing gradient gel electrophoresis (DGGE). Biomass in the column was heterogeneously distributed, with high levels of chemical oxygen demand, DNA and protein located near the inlet and a community structure that varied along its length. Microbial diversity was, at minimum, 34 members, with a number of organisms exhibiting close phylogenetic: relationships with known dechlorinators, in particular, <italic>Dehalococcoides ethenogenes, Trichlorobacter thiogenes</italic> and <italic>Dehalobacter restrictus</italic>. Furthermore, seven consortium members had their closest relatives detected in other environments containing chlorinated compounds. Based on DGGE and cloning of 16S rRNA genes and phylogenetic analysis dominant Bacterial taxa were deemed to be <italic> Dehalococcoides ethenogenes, Shewanella putrefaciens, Acetobacterium psammolithicum </italic>, a putative member of the <italic>Clostrideaceae</italic>, the <italic> Anaerobaculum thermoterrenum</italic> group, the CFB group, <italic>Trichlorobacter </italic>, and <italic>Pseudomonas putida</italic>.

ISBN: 0612533719Subjects--Topical Terms:

1017734
Biology, Microbiology.

Community analysis of an anaerobic tetrachloroethene (PCE) degrading bacterial consortium.
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