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Identification of intracellular calc...

Wang, Lin.

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Identification of intracellular calcium receptors (Inositol 1,4,5-trisphosphate and ryanodine) during maturation in bovine oocytes.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Identification of intracellular calcium receptors (Inositol 1,4,5-trisphosphate and ryanodine) during maturation in bovine oocytes./
作者:	Wang, Lin.
面頁冊數:	119 p.
附註:	Major Professor: Kenneth L. White.
Contained By:	Dissertation Abstracts International61-12B.
標題:	Agriculture, Animal Pathology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9999119
ISBN:	0493073183

Identification of intracellular calcium receptors (Inositol 1,4,5-trisphosphate and ryanodine) during maturation in bovine oocytes.
Wang, Lin.

Identification of intracellular calcium receptors (Inositol 1,4,5-trisphosphate and ryanodine) during maturation in bovine oocytes. - 119 p.

Major Professor: Kenneth L. White.

Thesis (Ph.D.)--Utah State University, 2000.

Intracellular calcium transients (oscillations) are critical to oocyte activation and subsequent development. Inositol 1,4,5-trisphosphate (IP3) receptor (IP3R) and ryanodine receptor (RyR) have been identified as two ligand-gated calcium channels that play a critical role in calcium release. Immature and mature bovine oocytes as well as fertilized eggs were used to evaluate the presence of the IP3R and the RyR as well as the spatial and temporal relationship of these receptors to endoplasmic reticulum (ER) and cortical granules (CG). Immunoprecipitation experiments were first carried out to verify the presence of these two receptors during early and late maturation using specific antibodies against these two receptors. Results indicate that IP3R is translated throughout the maturation period. In contrast, RyR is only translated during the late maturation period of bovine oocytes. The dynamic changes of RyR are due to the modification of protein synthesis rather than a conformational change during maturation of bovine oocytes.

ISBN: 0493073183Subjects--Topical Terms:

1021764
Agriculture, Animal Pathology.

Identification of intracellular calcium receptors (Inositol 1,4,5-trisphosphate and ryanodine) during maturation in bovine oocytes.
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245 1 0 $a Identification of intracellular calcium receptors (Inositol 1,4,5-trisphosphate and ryanodine) during maturation in bovine oocytes.
300 $a 119 p.
500 $a Major Professor: Kenneth L. White.
500 $a Source: Dissertation Abstracts International, Volume: 61-12, Section: B, page: 6242.
502 $a Thesis (Ph.D.)--Utah State University, 2000.
520 $a Intracellular calcium transients (oscillations) are critical to oocyte activation and subsequent development. Inositol 1,4,5-trisphosphate (IP3) receptor (IP3R) and ryanodine receptor (RyR) have been identified as two ligand-gated calcium channels that play a critical role in calcium release. Immature and mature bovine oocytes as well as fertilized eggs were used to evaluate the presence of the IP3R and the RyR as well as the spatial and temporal relationship of these receptors to endoplasmic reticulum (ER) and cortical granules (CG). Immunoprecipitation experiments were first carried out to verify the presence of these two receptors during early and late maturation using specific antibodies against these two receptors. Results indicate that IP3R is translated throughout the maturation period. In contrast, RyR is only translated during the late maturation period of bovine oocytes. The dynamic changes of RyR are due to the modification of protein synthesis rather than a conformational change during maturation of bovine oocytes.
520 $a Furthermore, immunocytochemistry, fluorescent staining, and laser scanning confocal microscopy were applied to reveal the localization of the IP3R and the RyR. The significant dynamics were observed in both IP3R and RyR at four different developmental stages from the germinal vesicle-intact stage (GV-intact) to 6 hours postinsemination (6 hpi). In addition, both the ER and the CG also showed apparent dynamic changes during meiotic maturation and at fertilization. Thus, based on the data, we proposed: (1) both IP3R and RyR contribute to the significant increase of intracellular calcium upon activation at fertilization, the dynamics of IP3R and RyR account for the increased intracellular calcium responsiveness during activation at fertilization; (2) the IP3R will associate with the ER, whereas the RyR will associate with the CG; (3) the IP3R and the RyR serve differently in calcium homeostasis and calcium release during meiotic maturation and at fertilization. In conclusion, both IP3R and RyR are present in bovine oocytes during maturation.
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