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Molecular and genetic studies on the...

Alfonso, Antonio Andres.

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Molecular and genetic studies on the restoration of fertility in cytoplasmic male sterile petunia.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Molecular and genetic studies on the restoration of fertility in cytoplasmic male sterile petunia./
作者:	Alfonso, Antonio Andres.
面頁冊數:	215 p.
附註:	Adviser: Maureen R. Hanson.
Contained By:	Dissertation Abstracts International63-12B.
標題:	Agriculture, Plant Culture. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3075805
ISBN:	0493960805

Molecular and genetic studies on the restoration of fertility in cytoplasmic male sterile petunia.
Alfonso, Antonio Andres.

Molecular and genetic studies on the restoration of fertility in cytoplasmic male sterile petunia. - 215 p.

Adviser: Maureen R. Hanson.

Thesis (Ph.D.)--Cornell University, 2003.

Cytoplasmic male sterility is caused by novel chimeric mitochondrial genes whose protein products interfere with microsporogenesis. Expression of CMS genes is suppressed by one or more nuclear fertility restorer genes, thereby allowing normal pollen production. The petunia CMS-associated locus called <italic>pcf</italic> encodes a 19.5 kD protein that is highly expressed in the tapetum of floral buds. A dominant nuclear gene called restorer of fertility (<italic>Rf</italic>) suppresses CMS by dramatically reducing PCF expression.

ISBN: 0493960805Subjects--Topical Terms:

1018669
Agriculture, Plant Culture.

Molecular and genetic studies on the restoration of fertility in cytoplasmic male sterile petunia.
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100 1 $a Alfonso, Antonio Andres. $3 1256345
245 1 0 $a Molecular and genetic studies on the restoration of fertility in cytoplasmic male sterile petunia.
300 $a 215 p.
500 $a Adviser: Maureen R. Hanson.
500 $a Source: Dissertation Abstracts International, Volume: 63-12, Section: B, page: 5667.
502 $a Thesis (Ph.D.)--Cornell University, 2003.
520 $a Cytoplasmic male sterility is caused by novel chimeric mitochondrial genes whose protein products interfere with microsporogenesis. Expression of CMS genes is suppressed by one or more nuclear fertility restorer genes, thereby allowing normal pollen production. The petunia CMS-associated locus called <italic>pcf</italic> encodes a 19.5 kD protein that is highly expressed in the tapetum of floral buds. A dominant nuclear gene called restorer of fertility (<italic>Rf</italic>) suppresses CMS by dramatically reducing PCF expression.
520 $a Two homologous candidate open reading frames (designated <italic>Orf591 </italic> and <italic>Orf592</italic> based on the number of predicted amino acids) found in a BIBAC clone identified from a genomic screen were each tested for their ability to restore fertility in CMS lines. Targeting assays using GFP fused to a putative targeting signal from <italic>Orf592</italic> confirmed that it carries a mitochondrial transit sequence. <italic>Agrobacterium</italic>-mediated transformation of Petunia CMS lines using <italic>Orf592</italic> caused fertility restoration in many primary transformants. A strong correlation in the presence of the transgene, reduced amount of PCF protein expression and fertility restoration was found in the primary transformants (T<sub>0</sub>) and in two T<sub>1 </sub> populations.
520 $a Both <italic>Orf591</italic> and <italic>Orf592</italic> DNA sequences contain pentatricopeptide (PPR) motifs; hence they were renamed <italic>Rf-PPR591 </italic> and <italic>Rf-PPR592</italic>, respectively. Unlike <italic>Rf-PPR592, Rf-PPR591</italic> did not restore fertility in over three dozen independent transformants. The recessive allele <italic>rf-PPR592</italic> had a 530-nt deletion in its promoter compared to the dominant allele <italic>Rf-PPR592 </italic>. Substitution of the promoter of <italic>Rf-PPR592</italic> with the promoter of <italic>rf-PPR592</italic> did not cause restoration in the transformants. Hence, the <italic>rf</italic> allele's inability to restore fertility is likely due to mutations in the coding region.
520 $a Transformation using the <italic>2x35S::Rf-PPR592</italic> transgene produced non-restored plants, probably because 35S promoter is poorly expressed in the tapetal cells. All the transgenes caused occasional abnormal phenotypes resembling meristem function mutants. These altered phenotypes may provide useful clues to the function of ancestral PPR genes or implicate the <italic> Rf</italic> gene in meristem development. Whether these phenotypes are the result of co-suppression or enhanced expression of the <italic>Rf</italic> gene or other endogenous genes remains to be determined.
590 $a School code: 0058.
650 4 $a Agriculture, Plant Culture. $3 1018669
650 4 $a Biology, Genetics. $3 1017730
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Biology, Plant Physiology. $3 1017865
690 $a 0307
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710 2 0 $a Cornell University. $3 530586
773 0 $t Dissertation Abstracts International $g 63-12B.
790 $a 0058
790 1 0 $a Hanson, Maureen R., $e advisor
791 $a Ph.D.
792 $a 2003
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3075805