語系:
繁體中文
English
說明(常見問題)
回圖書館首頁
手機版館藏查詢
登入
回首頁
切換:
標籤
|
MARC模式
|
ISBD
Introduction and characterization of...
~
Hahn, J. J.
FindBook
Google Book
Amazon
博客來
Introduction and characterization of the poly(3-hydroxybutyrate) biosynthetic pathway in plant cell cultures.
紀錄類型:
書目-語言資料,印刷品 : Monograph/item
正題名/作者:
Introduction and characterization of the poly(3-hydroxybutyrate) biosynthetic pathway in plant cell cultures./
作者:
Hahn, J. J.
面頁冊數:
248 p.
附註:
Adviser: Friedrich Srienc.
Contained By:
Dissertation Abstracts International59-03B.
標題:
Agriculture, Plant Culture. -
電子資源:
http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9826830
ISBN:
0591792664
Introduction and characterization of the poly(3-hydroxybutyrate) biosynthetic pathway in plant cell cultures.
Hahn, J. J.
Introduction and characterization of the poly(3-hydroxybutyrate) biosynthetic pathway in plant cell cultures.
- 248 p.
Adviser: Friedrich Srienc.
Thesis (Ph.D.)--University of Minnesota, 1998.
The expression in plants of biosynthetic pathways leading to the production of the commercially-valuable biodegradable polymer poly(3-hydroxybutyrate) (PHB) was investigated. For this purpose, the growth kinetics and nutrient uptake of Black Mexican Sweet (BMS) maize cell cultures (Zea mays L.) were thoroughly characterized both in a 2 L modified mammalian cell bioreactor and in shake flasks. Expression of the first two genes of the Ralstonia eutropha PHB biosynthetic pathway, encoding $\beta$-ketothiolase and acetoacetyl-CoA reductase, led to a slight increase in PHB precursor production and a significant decrease in growth rate. Each of the three genes of the Ralstonia eutropha PHB biosynthetic pathway was modified by the addition of a six amino acid transit peptide to the carboxy terminus in order to target expression to the peroxisome. This resulted in up to 0.4 mg/g fresh weight PHB when only the final gene of the pathway, encoding the PHA synthase enzyme, was expressed and up to 2 mg/g fresh weight PHB when all three genes of the pathway were expressed. In both cases, a slight decrease in culture growth rates was observed. The peroxisomal localization of PHB accumulation was verified by means of sucrose density gradient fractionation and freeze-fracture electron microscopy. Additional work involved the expression of the Pseudomonas oleovorans PHA Polymerase 2 gene in maize peroxisomes and the expression of the R. eutropha PHA synthase gene in the peroxisomes of intact tobacco and Arabidopsis thaliana plants. In tobacco (Nicotiana tabacum) plants, preliminary gas chromatography measurements indicate accumulation of PHB in leaves and roots of up to 3% of dry weight and in senescent leaves of up to 5% of dry weight. Metabolic modeling was used to predict PHBV copolymer composition, and a preliminary cost analysis was developed for a plant to co-produce corn starch and PHB from transgenic maize.
ISBN: 0591792664Subjects--Topical Terms:
1018669
Agriculture, Plant Culture.
Introduction and characterization of the poly(3-hydroxybutyrate) biosynthetic pathway in plant cell cultures.
LDR
:02864nam 2200289 a 45
001
928337
005
20110426
008
110426s1998 eng d
020
$a
0591792664
035
$a
(UnM)AAI9826830
035
$a
AAI9826830
040
$a
UnM
$c
UnM
100
1
$a
Hahn, J. J.
$3
1251798
245
1 0
$a
Introduction and characterization of the poly(3-hydroxybutyrate) biosynthetic pathway in plant cell cultures.
300
$a
248 p.
500
$a
Adviser: Friedrich Srienc.
500
$a
Source: Dissertation Abstracts International, Volume: 59-03, Section: B, page: 1231.
502
$a
Thesis (Ph.D.)--University of Minnesota, 1998.
520
$a
The expression in plants of biosynthetic pathways leading to the production of the commercially-valuable biodegradable polymer poly(3-hydroxybutyrate) (PHB) was investigated. For this purpose, the growth kinetics and nutrient uptake of Black Mexican Sweet (BMS) maize cell cultures (Zea mays L.) were thoroughly characterized both in a 2 L modified mammalian cell bioreactor and in shake flasks. Expression of the first two genes of the Ralstonia eutropha PHB biosynthetic pathway, encoding $\beta$-ketothiolase and acetoacetyl-CoA reductase, led to a slight increase in PHB precursor production and a significant decrease in growth rate. Each of the three genes of the Ralstonia eutropha PHB biosynthetic pathway was modified by the addition of a six amino acid transit peptide to the carboxy terminus in order to target expression to the peroxisome. This resulted in up to 0.4 mg/g fresh weight PHB when only the final gene of the pathway, encoding the PHA synthase enzyme, was expressed and up to 2 mg/g fresh weight PHB when all three genes of the pathway were expressed. In both cases, a slight decrease in culture growth rates was observed. The peroxisomal localization of PHB accumulation was verified by means of sucrose density gradient fractionation and freeze-fracture electron microscopy. Additional work involved the expression of the Pseudomonas oleovorans PHA Polymerase 2 gene in maize peroxisomes and the expression of the R. eutropha PHA synthase gene in the peroxisomes of intact tobacco and Arabidopsis thaliana plants. In tobacco (Nicotiana tabacum) plants, preliminary gas chromatography measurements indicate accumulation of PHB in leaves and roots of up to 3% of dry weight and in senescent leaves of up to 5% of dry weight. Metabolic modeling was used to predict PHBV copolymer composition, and a preliminary cost analysis was developed for a plant to co-produce corn starch and PHB from transgenic maize.
590
$a
School code: 0130.
650
4
$a
Agriculture, Plant Culture.
$3
1018669
650
4
$a
Biology, Molecular.
$3
1017719
650
4
$a
Engineering, Chemical.
$3
1018531
690
$a
0307
690
$a
0479
690
$a
0542
710
2 0
$a
University of Minnesota.
$3
676231
773
0
$t
Dissertation Abstracts International
$g
59-03B.
790
$a
0130
790
1 0
$a
Srienc, Friedrich,
$e
advisor
791
$a
Ph.D.
792
$a
1998
856
4 0
$u
http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=9826830
筆 0 讀者評論
館藏地:
全部
電子資源
出版年:
卷號:
館藏
1 筆 • 頁數 1 •
1
條碼號
典藏地名稱
館藏流通類別
資料類型
索書號
使用類型
借閱狀態
預約狀態
備註欄
附件
W9099799
電子資源
11.線上閱覽_V
電子書
EB W9099799
一般使用(Normal)
在架
0
1 筆 • 頁數 1 •
1
多媒體
評論
新增評論
分享你的心得
Export
取書館
處理中
...
變更密碼
登入