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Biological significance of bacterial...

Monier, Jean-Michel.

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Biological significance of bacterial aggregation on leaf surfaces: The social life of epiphytic bacteria.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Biological significance of bacterial aggregation on leaf surfaces: The social life of epiphytic bacteria./
作者:	Monier, Jean-Michel.
面頁冊數:	219 p.
附註:	Chair: Steven E. Lindow.
Contained By:	Dissertation Abstracts International63-09B.
標題:	Agriculture, Plant Pathology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3063495
ISBN:	0493823875

Biological significance of bacterial aggregation on leaf surfaces: The social life of epiphytic bacteria.
Monier, Jean-Michel.

Biological significance of bacterial aggregation on leaf surfaces: The social life of epiphytic bacteria. - 219 p.

Chair: Steven E. Lindow.

Thesis (Ph.D.)--University of California, Berkeley, 2002.

The presence of a majority of epiphytic bacteria in large aggregates on leaves has many implications for the behavior of cells within such structures and raises many questions as how they form, the benefit they confer to the colonists, and how they influence the degree of interaction between bacteria on leaves. The goal of this work was to quantify the frequency, size and localization of bacterial aggregates on leaf surfaces and determine their biological significance for epiphytic populations. The main focus of this work was the plant pathogenic bacterium <italic>Pseudomonas syringae </italic> pv. <italic>syringae</italic> strain B728a inoculated onto bean leaves. Bacteria genetically marked with genes conferring fluorescent proteins of different colors were visualized using epifluorescence microscopy directly on leaf surfaces. In addition cell viability was assessed using propidium iodide. We report that <italic>P. syringae</italic> cells can adapt to the leaf surface environment by reducing their cell size, and suggest that such a response is due to limited nutrient resources which are quite variable on leaves. Bacterial cells are not randomly distributed on the leaf surface but occur in a wide range of cluster sizes, ranging from single cells to over 10<super>4</super> cells per aggregate. Large cell aggregates are not frequent on a given leaf but often account for the majority of the cells present. Aggregates are preferentially associated with particular leaf structures such as glandular trichomes. While moist conditions on the leaf surface favors aggregate formation, aggregated cells are much more capable of tolerating environmental stresses on leaves than solitary cells and the preferential survival of cells in aggregates promotes a highly clustered spatial distribution of bacteria on leaf surfaces. While the presence of aggregates may either enhance or hinder the survival of immigrating bacteria, depending on the nature of their interactions, direct bacterial interactions between two populations is limited to only those few cells in direct contact. The fate of immigrant cells depends on both the nature of the leaf feature and the number of resident cells at their landing site. The infrequent occurrence of mixed species aggregates on leaves, and of infrequent contact of cells even within these mixed species aggregates, reveals that bacterial colonization of leaves is characterized by a high level of spatial segregation.

ISBN: 0493823875Subjects--Topical Terms:

1028950
Agriculture, Plant Pathology.

Biological significance of bacterial aggregation on leaf surfaces: The social life of epiphytic bacteria.
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