東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

Development of protein VII and prote...

Gao, Changshou.

FindBook

Google Book

Amazon

博客來

Development of protein VII and protein IX as the new platforms for phage display.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Development of protein VII and protein IX as the new platforms for phage display./
作者:	Gao, Changshou.
面頁冊數:	109 p.
附註:	Adviser: Kim D. Janda.
Contained By:	Dissertation Abstracts International63-08B.
標題:	Biology, Microbiology. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3061028
ISBN:	0493767444

Development of protein VII and protein IX as the new platforms for phage display.
Gao, Changshou.

Development of protein VII and protein IX as the new platforms for phage display. - 109 p.

Adviser: Kim D. Janda.

Thesis (Ph.D.)--The Scripps Research Institute, 2002.

This thesis comprises efforts toward the development of the minor coat proteins pVII and pIX of filamentous bacteriophage as new platforms for phage display, as well as the construction and investigation of a pIII-displayed scFv library. The research is described in four chapters.

ISBN: 0493767444Subjects--Topical Terms:

1017734
Biology, Microbiology.

Development of protein VII and protein IX as the new platforms for phage display.
LDR:03297nam 2200313 a 45 001 927784
005 20110425
008 110425s2002 eng d
020 $a 0493767444
035 $a (UnM)AAI3061028
035 $a AAI3061028
040 $a UnM $c UnM
100 1 $a Gao, Changshou. $3 1251347
245 1 0 $a Development of protein VII and protein IX as the new platforms for phage display.
300 $a 109 p.
500 $a Adviser: Kim D. Janda.
500 $a Source: Dissertation Abstracts International, Volume: 63-08, Section: B, page: 3587.
502 $a Thesis (Ph.D.)--The Scripps Research Institute, 2002.
520 $a This thesis comprises efforts toward the development of the minor coat proteins pVII and pIX of filamentous bacteriophage as new platforms for phage display, as well as the construction and investigation of a pIII-displayed scFv library. The research is described in four chapters.
520 $a In chapter one, the orientations of pVII and pIX were verified and demonstrated to be suitable for the display of a heterodimeric array using the antibody structure as a classic paradigm. The well-studied catalytic antibody PCP21H3 was used as an example and its variable regions of heavy chain and light chain were fused to the N-termini of pVII and pIX, respectively. Significantly, the phage-displayed Fv maintained fully functional binding and catalytic activities.
520 $a In chapters two and chapter three, the pVII and pIX technology was taken a step further so as to construct a novel random peptide library and a naïve human scFv library. The peptide library was panned against B-lymphocyte WI-L2 cells with the aim of isolating internalizing peptides. After five rounds of panning, one unique peptide-phage, designated CHL8, was identified that specifically bound to and penetrated the cells. The quality and utility of a naïve human scFv library of 4.5 × 10<super>9</super> members displayed on pIX was examined by panning against six different protein antigens. Specific antibodies against all six antigens were obtained and more than 90% of the clones showed positive binding for their respective antigens after as few as three rounds of panning. Furthermore, antibodies with nanomolar to subnanomolar affinity were directly isolated from this naïve human scFv library against antigens such as SEB and CTB.
520 $a In chapter four, a pIII phage-displayed naïve human scFv library was harnessed to isolate tumor-specific and internalizing antibodies by direct selection against tumor cell lines together with normal cell line subtraction. Three scFvs, SW1, PAN10, and PR5 were isolated from panning and their receptors were identified to be human integrin α3β1 (SW1, PAN10) and the transferrin receptor (PR5). The scFv SW1 was studied in further detail and found to induce functional effects as a ligand-mimetic by mediating cell adhesion and migration and the functional activities were integrin-α3 dependent.
590 $a School code: 1179.
650 4 $a Biology, Microbiology. $3 1017734
650 4 $a Biology, Molecular. $3 1017719
690 $a 0307
690 $a 0410
710 2 0 $a The Scripps Research Institute. $3 1251203
773 0 $t Dissertation Abstracts International $g 63-08B.
790 $a 1179
790 1 0 $a Janda, Kim D., $e advisor
791 $a Ph.D.
792 $a 2002
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3061028