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Ligand-receptor interactions in the ...

Wu, Chi-Hwa.

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Ligand-receptor interactions in the Wnt signaling pathway in Drosophila.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Ligand-receptor interactions in the Wnt signaling pathway in Drosophila./
Author:	Wu, Chi-Hwa.
Description:	203 p.
Notes:	Adviser: Roel Nusse.
Contained By:	Dissertation Abstracts International63-03B.
Subject:	Biology, Molecular. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3048640
ISBN:	0493630708

Ligand-receptor interactions in the Wnt signaling pathway in Drosophila.
Wu, Chi-Hwa.

Ligand-receptor interactions in the Wnt signaling pathway in Drosophila. - 203 p.

Adviser: Roel Nusse.

Thesis (Ph.D.)--Stanford University, 2002.

Wnts are a group of secreted proteins involved in cell to cell signaling. Studies in different organisms have indicated that Wnt proteins function in specifying cell fates during various developmental processes. Frizzled molecules are known to function as receptors for Wnt proteins on the cell surface. However, little is known about how Frizzleds transduce Wnts signals and how the signaling specificity is achieved.

ISBN: 0493630708Subjects--Topical Terms:

1017719
Biology, Molecular.

Ligand-receptor interactions in the Wnt signaling pathway in Drosophila.
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020 $a 0493630708
035 $a (UnM)AAI3048640
035 $a AAI3048640
040 $a UnM $c UnM
100 1 $a Wu, Chi-Hwa. $3 1251284
245 1 0 $a Ligand-receptor interactions in the Wnt signaling pathway in Drosophila.
300 $a 203 p.
500 $a Adviser: Roel Nusse.
500 $a Source: Dissertation Abstracts International, Volume: 63-03, Section: B, page: 1187.
502 $a Thesis (Ph.D.)--Stanford University, 2002.
520 $a Wnts are a group of secreted proteins involved in cell to cell signaling. Studies in different organisms have indicated that Wnt proteins function in specifying cell fates during various developmental processes. Frizzled molecules are known to function as receptors for Wnt proteins on the cell surface. However, little is known about how Frizzleds transduce Wnts signals and how the signaling specificity is achieved.
520 $a Seven <italic>Wnt</italic> genes and five <italic>frizzled</italic> genes were identified in the genome of <italic>Drosophila</italic>. Genetic experiments have revealed specific interactions but also redundancies between different <italic> Wnt</italic> and <italic>frizzled</italic> genes <italic>in vivo</italic>. To understand the similarities and differences of various Wnt-Frizzled signaling pathways, it is crucial to develop assays to determine ligand/receptor relationships and the strength of binding. Since no purified Wnt proteins are available to conduct binding experiments, I have established a reverse binding assay. This assay can be used to measure binding affinities between membrane-tethered ligands and soluble ligand binding domains of the receptors.
520 $a By using this reverse binding assay, I have determined binding affinities between various <italic>Drosophila</italic> Wnt and Frizzled molecules. The biological significance of these interactions is also discussed here. While more <italic>in vivo</italic> experiments are in progress, combining the results from genetics and the data generated in these binding assays will shed more light on how ligand-receptor interactions regulate Wnt signalings.
520 $a In a different set of experiments, I addressed whether there are separate domains in Wnt proteins required for activity and how Wnt proteins are folded. By using an <italic>in vitro</italic> activity assay for Wingless proteins, I tested secretion and activity of the amino-terminal region and the carboxyl-terminal region of Wingless. This assay suggested that Wingless might contain separate folding domains. Furthermore, both the amino-terminal and the carboxyl-terminal region of Wingless are required for activity.
590 $a School code: 0212.
650 4 $a Biology, Molecular. $3 1017719
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710 2 0 $a Stanford University. $3 754827
773 0 $t Dissertation Abstracts International $g 63-03B.
790 $a 0212
790 1 0 $a Nusse, Roel, $e advisor
791 $a Ph.D.
792 $a 2002
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3048640