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Functions of nuclear inner membrane ...

Lee, Kenneth K.

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Functions of nuclear inner membrane proteins in humans and Caenorhabditis elegans.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Functions of nuclear inner membrane proteins in humans and Caenorhabditis elegans./
作者:	Lee, Kenneth K.
面頁冊數:	218 p.
附註:	Adviser: Katherine L. Wilson.
Contained By:	Dissertation Abstracts International63-03B.
標題:	Biology, Cell. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3046491
ISBN:	0493606815

Functions of nuclear inner membrane proteins in humans and Caenorhabditis elegans.
Lee, Kenneth K.

Functions of nuclear inner membrane proteins in humans and Caenorhabditis elegans. - 218 p.

Adviser: Katherine L. Wilson.

Thesis (Ph.D.)--The Johns Hopkins University, 2002.

Proteins of the inner nuclear membrane are fundamentally important for nuclear structure and function. Three such proteins, named LAP2, emerin and MAN1, share the 43-residue ‘LEM’ domain. Loss of emerin causes Emery-Dreifuss muscular dystrophy (EDMD), in humans, through unknown mechanisms. To understand this disease, I characterized the functions of human emerin. I first tested the hypothesis that emerin binds barrier-to-autointegration factor (BAF), an essential, conserved DNA-bridging protein in multicellular eukaryotes, with potential roles in nuclear assembly and chromatin organization. Binding was tested biochemically, and domains mapped using 17 alanine-substitution mutants of emerin. This work identified two functional domains in human emerin: the LEM-domain, which is required to bind BAF, and a central domain required to bind lamin A. These results are significant; they suggest that BAF is a binding partner for all LEM-domain proteins, and led me to discover that two disease-causing mutations map outside the BAF- and lamin A-binding regions, potentially defining additional or unique functional domains in emerin that relate directly to the EDMD disease mechanism. I also used <italic>C. elegans </italic> to determine if emerin function is conserved in a genetically tractable system. After identifying and cloning the three LEM-domain genes in <italic> C. elegans</italic>, I showed that the biochemical properties, localization and loss-of-function phenotype of <italic>C. elegans</italic> emerin parallel human emerin. I am currently determining which biochemical functions of human emerin are shared with <italic>C. elegans</italic> emerin, for future genetic analysis of emerin function.

ISBN: 0493606815Subjects--Topical Terms:

1017686
Biology, Cell.

Functions of nuclear inner membrane proteins in humans and Caenorhabditis elegans.
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