東華大學圖書館 |

Language: English

Help

回圖書館首頁

手機版館藏查詢

Back

Switch To: Labeled | MARC Mode | ISBD

Actin polymerization in gene expression.

Rando, Oliver Joaquin.

Linked to FindBook

Google Book

Amazon

博客來

Actin polymerization in gene expression.

Record Type:	Language materials, printed : Monograph/item
Title/Author:	Actin polymerization in gene expression./
Author:	Rando, Oliver Joaquin.
Description:	182 p.
Notes:	Adviser: Gerald R. Crabtree.
Contained By:	Dissertation Abstracts International63-01B.
Subject:	Biology, Cell. -
Online resource:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3040059
ISBN:	0493533842

Actin polymerization in gene expression.
Rando, Oliver Joaquin.

Actin polymerization in gene expression. - 182 p.

Adviser: Gerald R. Crabtree.

Thesis (Ph.D.)--Stanford University, 2002.

In order to better understand the mechanism of action of Swi/Snf-like chromatin remodeling complexes, we investigated the role of actin and the actin-related BAF53 in the human Swi/Snf-like BAF complex. Here, we present evidence that actin and BAF53 are involved in targeting of the BAF complex to the nuclear scaffold. We show that this scaffold association can be regulated by a number of signaling mediators, including the lipid phosphatidylinositol 4,5-bisphosphate (PIP2). We further show that binding of PIP 2 to the BAF complex allows the BAF complex to stabilize actin filaments by binding to filament ends and filament branch points, and suggest a mechanism for this regulation. In order to identify potential target genes of BAF function in vivo, we used cDNA microarrays to interrogate gene expression changes in peripheral T-cells and in the Jurkat T-cell line under a variety of conditions. We identify genes whose activation in Jurkats requires both actin polymerization as well as an intact BAF complex. In addition, we present results of studies on gene expression in T-cells in response to activation and in response to perturbations in the cytoskeleton.

ISBN: 0493533842Subjects--Topical Terms:

1017686
Biology, Cell.

Actin polymerization in gene expression.
LDR:02059nam 2200289 a 45 001 927675
005 20110425
008 110425s2002 eng d
020 $a 0493533842
035 $a (UnM)AAI3040059
035 $a AAI3040059
040 $a UnM $c UnM
100 1 $a Rando, Oliver Joaquin. $3 1251239
245 1 0 $a Actin polymerization in gene expression.
300 $a 182 p.
500 $a Adviser: Gerald R. Crabtree.
500 $a Source: Dissertation Abstracts International, Volume: 63-01, Section: B, page: 0130.
502 $a Thesis (Ph.D.)--Stanford University, 2002.
520 $a In order to better understand the mechanism of action of Swi/Snf-like chromatin remodeling complexes, we investigated the role of actin and the actin-related BAF53 in the human Swi/Snf-like BAF complex. Here, we present evidence that actin and BAF53 are involved in targeting of the BAF complex to the nuclear scaffold. We show that this scaffold association can be regulated by a number of signaling mediators, including the lipid phosphatidylinositol 4,5-bisphosphate (PIP2). We further show that binding of PIP 2 to the BAF complex allows the BAF complex to stabilize actin filaments by binding to filament ends and filament branch points, and suggest a mechanism for this regulation. In order to identify potential target genes of BAF function in vivo, we used cDNA microarrays to interrogate gene expression changes in peripheral T-cells and in the Jurkat T-cell line under a variety of conditions. We identify genes whose activation in Jurkats requires both actin polymerization as well as an intact BAF complex. In addition, we present results of studies on gene expression in T-cells in response to activation and in response to perturbations in the cytoskeleton.
590 $a School code: 0212.
650 4 $a Biology, Cell. $3 1017686
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Biophysics, General. $3 1019105
690 $a 0307
690 $a 0379
690 $a 0786
710 2 0 $a Stanford University. $3 754827
773 0 $t Dissertation Abstracts International $g 63-01B.
790 $a 0212
790 1 0 $a Crabtree, Gerald R., $e advisor
791 $a Ph.D.
792 $a 2002
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3040059