東華大學圖書館 |

語系: 繁體中文

說明(常見問題)

回圖書館首頁

手機版館藏查詢

登入

回首頁

切換: 標籤 | MARC模式 | ISBD

Genetic analysis of morphogenesis in...

Gillmor, Charles Stewart, III.

FindBook

Google Book

Amazon

博客來

Genetic analysis of morphogenesis in the Arabidopsis embryo.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Genetic analysis of morphogenesis in the Arabidopsis embryo./
作者:	Gillmor, Charles Stewart, III.
面頁冊數:	163 p.
附註:	Adviser: Christopher R. Somerville.
Contained By:	Dissertation Abstracts International63-01B.
標題:	Biology, Botany. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3040019
ISBN:	049353265X

Genetic analysis of morphogenesis in the Arabidopsis embryo.
Gillmor, Charles Stewart, III.

Genetic analysis of morphogenesis in the Arabidopsis embryo. - 163 p.

Adviser: Christopher R. Somerville.

Thesis (Ph.D.)--Stanford University, 2002.

In a genetic screen for mutations that affect the morphology of the early <italic> Arabidopsis</italic> embryo, 6,000 embryo defective mutations was examined. Of these, about 300 had recognizable defects in cell elongation or cell division. Mutations in the <italic>VACUOLELESS1, KNOPF</italic>, and <italic>RADIALLY SWOLLEN1</italic> genes are described in this thesis.

ISBN: 049353265XSubjects--Topical Terms:

1017825
Biology, Botany.

Genetic analysis of morphogenesis in the Arabidopsis embryo.
LDR:03379nam 2200313 a 45 001 927672
005 20110425
008 110425s2002 eng d
020 $a 049353265X
035 $a (UnM)AAI3040019
035 $a AAI3040019
040 $a UnM $c UnM
100 1 $a Gillmor, Charles Stewart, III. $3 1251236
245 1 0 $a Genetic analysis of morphogenesis in the Arabidopsis embryo.
300 $a 163 p.
500 $a Adviser: Christopher R. Somerville.
500 $a Source: Dissertation Abstracts International, Volume: 63-01, Section: B, page: 0031.
502 $a Thesis (Ph.D.)--Stanford University, 2002.
520 $a In a genetic screen for mutations that affect the morphology of the early <italic> Arabidopsis</italic> embryo, 6,000 embryo defective mutations was examined. Of these, about 300 had recognizable defects in cell elongation or cell division. Mutations in the <italic>VACUOLELESS1, KNOPF</italic>, and <italic>RADIALLY SWOLLEN1</italic> genes are described in this thesis.
520 $a <italic>vacuoleless1</italic> mutants were identified by their severe defect in cell division orientation and cell elongation, beginning with the first stages of embryogenesis. The lack of vacuoles in all cells of <italic> vacuoleless1</italic> embryos leads to a severe, early embryo arrest phenotype. Autophagosomes accumulate in all cells of the embryo, and in the absence of a vacuole, large amounts of cellular material are abnormally secreted into the cell wall. The <italic>VACUOLELESS1</italic> gene was cloned and shown to encode a homolog of the yeast protein Vps16p, which is also required for vacuole biogenesis in yeast, where it is necessary for the docking and fusion of prevacuolar vesicles.
520 $a <italic>knopf</italic> and <italic>radially swollen 1</italic> (<italic> rsw1</italic>) mutants were identified based on their radially swollen phenotype during embryogenesis. The <italic>RSW1</italic> gene has previously been shown to encode a catalytic subunit of cellulose synthase. Mutations in both the <italic>KNOPF</italic> and <italic>RSW1</italic> genes decrease cellulose production in the embryo to less than 20% of that found in wild-type embryos, and both show altered cell elongation in the embryo. We show that <italic> KNOPF</italic> encodes α-glucosidase I, an ER-localized enzyme that trims the terminal α-1,2 linked glucose from asparagine-linked (N-linked) glycans. N-linked glycans are known to be required for ER-localized chaperone assisted folding of certain proteins, as well as enzyme activity. Using a cellulose synthase (CESA) antibody on protein extracts of <italic>knopf</italic> embryos, it was shown that the <italic>knopf</italic> mutation does not affect stability of CESA proteins. These results suggest that the requirement of N-glycosylation for cellulose biosynthesis is not in the CESA catalytic subunit, but in other enzyme(s) necessary for cellulose biosynthesis. In addition, our results demonstrate that loss of function mutations in genes required for cell wall biosynthesis can be recovered during embryogenesis.
590 $a School code: 0212.
650 4 $a Biology, Botany. $3 1017825
650 4 $a Biology, Cell. $3 1017686
650 4 $a Biology, Genetics. $3 1017730
690 $a 0309
690 $a 0369
690 $a 0379
710 2 0 $a Stanford University. $3 754827
773 0 $t Dissertation Abstracts International $g 63-01B.
790 $a 0212
790 1 0 $a Somerville, Christopher R., $e advisor
791 $a Ph.D.
792 $a 2002
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3040019