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Molecular guidance cues and intracel...

Starz-Gaiano, Michelle Ann.

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Molecular guidance cues and intracellular signaling in Drosophila germ cell migration.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Molecular guidance cues and intracellular signaling in Drosophila germ cell migration./
作者:	Starz-Gaiano, Michelle Ann.
面頁冊數:	229 p.
附註:	Adviser: Ruth Lehmann.
Contained By:	Dissertation Abstracts International63-08B.
標題:	Biology, Cell. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3062846
ISBN:	049380966X

Molecular guidance cues and intracellular signaling in Drosophila germ cell migration.
Starz-Gaiano, Michelle Ann.

Molecular guidance cues and intracellular signaling in Drosophila germ cell migration. - 229 p.

Adviser: Ruth Lehmann.

Thesis (Ph.D.)--New York University, 2002.

The migration of primordial germ cells provides an excellent model system for the study of cellular movements and signaling during development. Germ cells in the Drosophila embryo are set aside early in development and must invade tissues, seek out somatic support cells, and change adhesive property to coalesce into the embryonic gonad. The Gal4-UAS transcriptional activation system has allowed us to overexpress genes of interest specifically in the germ cells. A misexpression screen using this system identified two genes that can dramatically affect germ cell migration. One of these genes is a homolog of a gene known to be involved in germ cell migration, <italic>wunen </italic>. Loss-of-function results show that <italic>wunen</italic> and <italic> wunen-2</italic> cooperate to guide the germ cells by acting as repellants. These genes are fly homologs of mammalian phosphatidic acid phosphohydrolases (PAP2), and we demonstrate that the catalytic activity of Wunen 2 is required for its repellant function.

ISBN: 049380966XSubjects--Topical Terms:

1017686
Biology, Cell.

Molecular guidance cues and intracellular signaling in Drosophila germ cell migration.
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245 1 0 $a Molecular guidance cues and intracellular signaling in Drosophila germ cell migration.
300 $a 229 p.
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500 $a Source: Dissertation Abstracts International, Volume: 63-08, Section: B, page: 3550.
502 $a Thesis (Ph.D.)--New York University, 2002.
520 $a The migration of primordial germ cells provides an excellent model system for the study of cellular movements and signaling during development. Germ cells in the Drosophila embryo are set aside early in development and must invade tissues, seek out somatic support cells, and change adhesive property to coalesce into the embryonic gonad. The Gal4-UAS transcriptional activation system has allowed us to overexpress genes of interest specifically in the germ cells. A misexpression screen using this system identified two genes that can dramatically affect germ cell migration. One of these genes is a homolog of a gene known to be involved in germ cell migration, <italic>wunen </italic>. Loss-of-function results show that <italic>wunen</italic> and <italic> wunen-2</italic> cooperate to guide the germ cells by acting as repellants. These genes are fly homologs of mammalian phosphatidic acid phosphohydrolases (PAP2), and we demonstrate that the catalytic activity of Wunen 2 is required for its repellant function.
520 $a The second gene identified encodes a putative G protein-coupled receptor (GPCR) which, when overexpressed in the germ cells, causes them to become super-migratory. The GPCR transcript is dynamically expressed in migratory cell types in the embryo, including hemocytes, the midgut, and glia. Loss-of-function mutations in this gene do not reveal an essential role for it in germ cell migration, but this may be due to redundancy or maternal contribution of the protein. These overexpression results led us to investigate the involvement for other GPCRs and downstream signaling components, and this work established a role for the monomeric GTPase Rac in germ cell migration.
520 $a We propose that spatially restricted phospholipid hydrolysis creates a gradient of signal necessary and specific for the migration and survival of germ cells. Furthermore, based on work presented here and studies on the vertebrate Edg receptors, we postulate that a G protein-coupled receptor acts in the germ cells to translate phospholipid cues into directed migration, probably via cytoskeletal rearrangements orchestrated by Rac. Future work may identify additional components of this signaling cascade in the germ cells of flies and other organisms.
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