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Characterization of the heterochroni...

Jeon, Mili.

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Characterization of the heterochronic gene lin-42 and functional studies of the timeless-like gene tim-1 in Caenorhabditis elegans.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Characterization of the heterochronic gene lin-42 and functional studies of the timeless-like gene tim-1 in Caenorhabditis elegans./
作者:	Jeon, Mili.
面頁冊數:	142 p.
附註:	Major Adviser: Ann E. Rougvie.
Contained By:	Dissertation Abstracts International62-12B.
標題:	Biology, Cell. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3037475
ISBN:	0493505938

Characterization of the heterochronic gene lin-42 and functional studies of the timeless-like gene tim-1 in Caenorhabditis elegans.
Jeon, Mili.

Characterization of the heterochronic gene lin-42 and functional studies of the timeless-like gene tim-1 in Caenorhabditis elegans. - 142 p.

Major Adviser: Ann E. Rougvie.

Thesis (Ph.D.)--University of Minnesota, 2002.

The <italic>C. elegans</italic> heterochronic gene pathway controls the timing of many stage-specific events during the postembryonic development of the nematode, including the restriction of the timing of seam cell terminal differentiation to the L4-to-adult molt. Loss-of-function mutations in <italic> lin-42</italic> cause seam cells to terminally differentiate at the L3-to-L4 molt, one stage earlier than wild-type animals. The first part of my thesis concentrates on the molecular characterization of <italic>lin-42</italic>. LIN-42 is most similar to the PERIOD (PER) family of circadian rhythm proteins. A database search indicates that in the <italic>C. elegans</italic> genome, LIN-42 is the best match to PER. Remarkably, expression studies carried out by Heather Gardner in the lab showed that <italic>lin-42</italic> mRNA levels oscillate during postembryonic development. An oscillating expression pattern is a hallmark feature of circadian rhythm regulators such as <italic>per</italic>, whose message and protein levels oscillate with a 24-hour period in response to light-dark cycles. <italic>lin-42</italic> message level oscillation is not circadian, but is relative to the molting cycle that occurs with a 6-hour period during postembryonic development.

ISBN: 0493505938Subjects--Topical Terms:

1017686
Biology, Cell.

Characterization of the heterochronic gene lin-42 and functional studies of the timeless-like gene tim-1 in Caenorhabditis elegans.
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245 1 0 $a Characterization of the heterochronic gene lin-42 and functional studies of the timeless-like gene tim-1 in Caenorhabditis elegans.
300 $a 142 p.
500 $a Major Adviser: Ann E. Rougvie.
500 $a Source: Dissertation Abstracts International, Volume: 62-12, Section: B, page: 5507.
502 $a Thesis (Ph.D.)--University of Minnesota, 2002.
520 $a The <italic>C. elegans</italic> heterochronic gene pathway controls the timing of many stage-specific events during the postembryonic development of the nematode, including the restriction of the timing of seam cell terminal differentiation to the L4-to-adult molt. Loss-of-function mutations in <italic> lin-42</italic> cause seam cells to terminally differentiate at the L3-to-L4 molt, one stage earlier than wild-type animals. The first part of my thesis concentrates on the molecular characterization of <italic>lin-42</italic>. LIN-42 is most similar to the PERIOD (PER) family of circadian rhythm proteins. A database search indicates that in the <italic>C. elegans</italic> genome, LIN-42 is the best match to PER. Remarkably, expression studies carried out by Heather Gardner in the lab showed that <italic>lin-42</italic> mRNA levels oscillate during postembryonic development. An oscillating expression pattern is a hallmark feature of circadian rhythm regulators such as <italic>per</italic>, whose message and protein levels oscillate with a 24-hour period in response to light-dark cycles. <italic>lin-42</italic> message level oscillation is not circadian, but is relative to the molting cycle that occurs with a 6-hour period during postembryonic development.
520 $a In flies, TIMELESS (dTIM) physically associates with PER, and is an essential component of the molecular oscillator that generates circadian rhythms. Given the similarity of LIN-42 to PER, I investigated the role of the <italic>timeless </italic>-like protein TIM-1 in <italic>C. elegans</italic>. I describe the isolation of a mutation in <italic>tim-1</italic>. Phenotypic analysis of animals depleted of TIM-1 activity suggests that TIM-1 is required for proper chromosome segregation. Immunolocalization studies showed that TIM-1 expression pattern is consistent with its role in chromosome segregation. Our understanding of TIM-1 function, and analysis of sequence similarities between TIM-related proteins have led us to conclude that TIM-1 belongs to a second class of <italic> timeless</italic> proteins whose function is distinct from that of the clock-relevant dTIM protein.
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