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Membrane and microsparging aerations...

Qi, Hanshi Nianmin.

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Membrane and microsparging aerations in long-term high-density perfusion cultures of animal cells.

紀錄類型:	書目-語言資料,印刷品 : Monograph/item
正題名/作者:	Membrane and microsparging aerations in long-term high-density perfusion cultures of animal cells./
作者:	Qi, Hanshi Nianmin.
面頁冊數:	152 p.
附註:	Source: Dissertation Abstracts International, Volume: 63-03, Section: B, page: 1462.
Contained By:	Dissertation Abstracts International63-03B.
標題:	Biology, Cell. -
電子資源:	http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3044348
ISBN:	0493583874

Membrane and microsparging aerations in long-term high-density perfusion cultures of animal cells.
Qi, Hanshi Nianmin.

Membrane and microsparging aerations in long-term high-density perfusion cultures of animal cells. - 152 p.

Source: Dissertation Abstracts International, Volume: 63-03, Section: B, page: 1462.

Thesis (Ph.D.)--Oregon State University, 2002.

The effect of high superficial velocity of sparging gas on cells at the sparger surface proved insignificant.

ISBN: 0493583874Subjects--Topical Terms:

1017686
Biology, Cell.

Membrane and microsparging aerations in long-term high-density perfusion cultures of animal cells.
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245 1 0 $a Membrane and microsparging aerations in long-term high-density perfusion cultures of animal cells.
300 $a 152 p.
500 $a Source: Dissertation Abstracts International, Volume: 63-03, Section: B, page: 1462.
502 $a Thesis (Ph.D.)--Oregon State University, 2002.
520 $a The effect of high superficial velocity of sparging gas on cells at the sparger surface proved insignificant.
520 $a The profile of the inner-tubing gas pressure for a tubular membrane aeration system was quantified. The correlations among the overall volumetric oxygen transfer coefficient (kLa), the inner-tubing pressure, the tubing tightness, and the gas throughput are experimentally analyzed. A mathematical model was developed to describe the underlying phenomena. The results established the base for comparison with other aeration techniques.
520 $a A novel method employing <italic>in situ</italic> laser imaging technology to monitor bubbles and cells, and analyze bubble size distributions in a micro-sparged bioreactor was developed. The effects of bioreactor operations on bubble size distributions were determined with following results: (1) Spargers with larger pores produced larger bubbles in most cases; (2) Higher sparging rates resulted in bubble size increases up to 10%; (3) Pluronic F68 shrank bubbles up to 30%. When the concentration of Pluronic F68 exceeded 1 g/L, no additional impact was observed; (4) Emulsion silicone antifoam up to 25 ppm had no impact on bubbles; (5) Cell density (up to 22 × 10<super>6</super> cells/mL) or culture age has no effect on bubble sizes.
520 $a In multiple 15-L long-term high-density cultures of animal cells, the correlations between sparging rate and cell damage for using 0.5 μm and 15 μm-pore spargers were quantified. At cell density of 2 × 10<super> 7</super> cells/mL, sparging above 0.025 vvm using the 0.5-μm sparger was detrimental to cells, while 0.054 vvm was detrimental for the 15-μm sparger. A model was developed to predict the rate of cell death resulted from cell-bubble interactions for high-density industrial animal cell cultures.
520 $a A new dissolved CO2 sensor proved to be reliable for long-term use in industrial perfusion cell cultures. A novel method for the control of dissolved CO2 while simultaneously maintaining DO2 and pH setpoints was developed. The continuous control of dissolved CO 2, DO2 and pH is achieved by simultaneously adjusting the total sparging rate as well as the ratio of O2, N2 and CO2 gas contents. This control strategy enables optimization of dissolved CO2 in industrial culture processes and allows for improved cell growth and protein production.
590 $a School code: 0172.
650 4 $a Biology, Cell. $3 1017686
650 4 $a Biology, Molecular. $3 1017719
650 4 $a Engineering, Biomedical. $3 1017684
650 4 $a Engineering, Chemical. $3 1018531
690 $a 0307
690 $a 0379
690 $a 0541
690 $a 0542
710 2 0 $a Oregon State University. $3 625720
773 0 $t Dissertation Abstracts International $g 63-03B.
790 $a 0172
791 $a Ph.D.
792 $a 2002
856 4 0 $u http://pqdd.sinica.edu.tw/twdaoapp/servlet/advanced?query=3044348